Flow cytometry analysis of alpha(1)-adrenoceptor subtypes

被引：18

作者：

Hirasawa, A

Tsumaya, K

Awaji, T

Shibata, K

Homma, N

Shinomiya, T

Tsujimoto, G

机构：

[1] NATL CHILDRENS MED RES CTR,DEPT MOL CELL PHARMACOL,SETAGAYA KU,TOKYO 154,JAPAN

[2] NATL CHILDRENS MED RES CTR,DEPT EXPTL SURG,SETAGAYA KU,TOKYO 154,JAPAN

来源：

FEBS LETTERS | 1996年 / 386卷 / 2-3期

关键词：

alpha-adrenoceptor; BODIPY-FL prazosin; antipeptide antibody; flow cytometry; hamster DDT1-MF2 cell;

D O I：

10.1016/0014-5793(96)00388-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To characterize the alpha(1)-adrenoceptor subtypes, we developed a flow cytometry method using the fluorescent ligand BODIPY-FL prazosin and the anti-peptide antibody against the alpha(1b)-adrenoceptor amino terminus (designated 1B-N1-C) as probes, Three alpha(1)-adrenoceptors (alpha(1a), alpha(1b) and alpha(1d)) expressed in CHO cells were detected by BODIPY-FL prazosin; however, only alpha(1b)-adrenoceptor subtype was detected by the anti-peptide antibody 1B-N1-C, Furthermore, the flow cytometry analysis with 1B-N1-C specifically identified alpha(1b)-adrenoceptor in native cells of hamster DDT1-MF2 cells, rat hepatocytes and1 cardiomyocytes.

引用

收藏

页码：141 / 148

页数：8

相关论文

共 28 条

[1] MOLECULAR-CLONING AND EXPRESSION OF THE CDNA FOR THE HAMSTER ALPHA-1-ADRENERGIC RECEPTOR [J].

COTECCHIA, S ;

SCHWINN, DA ;

RANDALL, RR ;

LEFKOWITZ, RJ ;

CARON, MG ;

KOBILKA, BK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7159-7163

[2]

ESBENSHADE TA, 1995, MOL PHARMACOL, V47, P977

[3] USE OF RECOMBINANT ALPHA(1)-ADRENOCEPTORS TO CHARACTERIZE SUBTYPE SELECTIVITY OF DRUGS FOR THE TREATMENT OF PROSTATIC HYPERTROPHY [J].

FOGLAR, R ;

SHIBATA, K ;

HORIE, K ;

HIRASAWA, A ;

TSUJIMOTO, G .

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1995, 288 (02) :201-207

[4] ALPHA-1-ADRENOCEPTOR SUBTYPES LINKED TO DIFFERENT MECHANISMS FOR INCREASING INTRACELLULAR CA-2+ IN SMOOTH-MUSCLE [J].

HAN, C ;

ABEL, PW ;

MINNEMAN, KP .

NATURE, 1987, 329 (6137) :333-335

[5] SYNTHESIS AND PROPERTIES OF FLUORESCENT BETA-ADRENOCEPTOR LIGANDS [J].

HEITHIER, H ;

HALLMANN, D ;

BOEGE, F ;

REILANDER, H ;

DEES, C ;

JAEGGI, KA ;

ARNDTJOVIN, D ;

JOVIN, TM ;

HELMREICH, EJM .

BIOCHEMISTRY, 1994, 33 (31) :9126-9134

[6] 2 DISTINCT ENHANCERS WITH DIFFERENT CELL SPECIFICITIES COEXIST IN THE REGULATORY REGION OF POLYOMA [J].

HERBOMEL, P ;

BOURACHOT, B ;

YANIV, M .

CELL, 1984, 39 (03) :653-662

[7] CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF HUMAN ALPHA(1C)-ADRENOCEPTOR SPLICE VARIANTS [J].

HIRASAWA, A ;

SHIBATA, K ;

HORIE, K ;

TAKEI, Y ;

OBIKA, K ;

TANAKA, T ;

MURAMOTO, N ;

TAKAGAKI, K ;

YANO, J ;

TSUJIMOTO, G .

FEBS LETTERS, 1995, 363 (03) :256-260

[8] CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF HUMAN CDNA FOR THE ALPHA(1C)-ADRENERGIC RECEPTOR [J].

HIRASAWA, A ;

HORIE, K ;

TANAKA, T ;

TAKAGAKI, K ;

MURAI, M ;

YANO, J ;

TSUJIMOTO, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 195 (02) :902-909

[9] THE PHARMACOLOGICAL PROFILE OF CLONED AND STABLY EXPRESSED ALPHA(1B)-ADRENOCEPTOR IN CHO CELLS [J].

HORIE, K ;

HIRASAWA, A ;

TSUJIMOTO, G .

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION, 1994, 268 (03) :399-407

[10] CALCIUM TOLERANT VENTRICULAR MYOCYTES PREPARED BY PRE-INCUBATION IN A KB MEDIUM [J].

ISENBERG, G ;

KLOCKNER, U .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1982, 395 (01) :6-18