Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice

被引：37

作者：

Dong, Qing ^{[1
,2
]}

Xu, Qianqian ^{[1
]}

Kong, Jingjing ^{[1
]}

Peng, Xiaojian ^{[1
]}

Zhou, Wei ^{[1
]}

Chen, Long ^{[1
]}

Wu, Jiandong ^{[1
]}

Xiang, Yan ^{[1
]}

Jiang, Haiyang ^{[1
]}

Cheng, Beijiu ^{[1
]}

机构：

[1] Anhui Agr Univ, Natl Engn Lab Crop Stress Resistence, Hefei 230036, Anhui, Peoples R China

[2] Anhui Acad Agr Sci, Maize Res Ctr, Hefei 230031, Anhui, Peoples R China

来源：

PLANT SCIENCE | 2019年 / 283卷

基金：

中国国家自然科学基金; 安徽省自然科学基金;

关键词：

ZmbZIP22; Transcription; Endosperm; Regulation; Amylose; Biosynthesis; ZIPPER TRANSCRIPTION FACTOR; GENOME-WIDE ANALYSIS; DEBRANCHING ENZYME; GRAIN-SIZE; PROTEIN; MUTANTS; AMYLOSE; BIOSYNTHESIS; PULLULANASE; ARABIDOPSIS;

D O I：

10.1016/j.plantsci.2019.03.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Starch content and composition are major determinants of yield and quality in maize. In recent years, the major genes for starch metabolism have been cloned in this species. However, the role of transcription factors in regulating the starch metabolism pathway remains unclear. The ZmbZIP22 gene encodes a bZIP transcription factor. In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules. Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize. ZmbZIP22 promotes the transcription of starch metabolism genes by binding to their promoters. Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes. Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.

引用

页码：407 / 415

页数：9

共 44 条

[1] Cloning of cDNA for UDP-glucose pyrophosphorylase and the expression of mRNA in rice endosperm [J].

Abe, T ;

Niiyama, H ;

Sasahara, T .

THEORETICAL AND APPLIED GENETICS, 2002, 105 (2-3) :216-221

[2] AN ARGININE TO LYSINE SUBSTITUTION IN THE BZIP DOMAIN OF AN OPAQUE-2 MUTANT IN MAIZE ABOLISHES SPECIFIC DNA-BINDING [J].

AUKERMAN, MJ ;

SCHMIDT, RJ ;

BURR, B ;

BURR, FA .

GENES & DEVELOPMENT, 1991, 5 (02) :310-320

[3] Identification of Mutator insertional mutants of starch-branching enzyme 1 (sbe1) in Zea mays L. [J].

Blauth S.L. ;

Kim K.-N. ;

Klucinec J. ;

Shannon J.C. ;

Thompson D. ;

Guiltinan M. .

Plant Molecular Biology, 2002, 48 (03) :287-297

[4] Starch granule initiation and growth are altered in barley mutants that lack isoamylase activity [J].

Burton, RA ;

Jenner, H ;

Carrangis, L ;

Fahy, B ;

Fincher, GB ;

Hylton, C ;

Laurie, DA ;

Parker, M ;

Waite, D ;

van Wegen, S ;

Verhoeven, T ;

Denyer, K .

PLANT JOURNAL, 2002, 31 (01) :97-112

[5] Rice bZIP protein, REB, interacts with GCN4 motif in promoter of Waxy gene [J].

Cheng S. ;

Wang Z. ;

Hong M. .

Science in China, Series C: Life Sciences, 2002, 45 (04) :352-360

[6] RF2b, a rice bZIP transcription activator, interacts with RF2a and is involved in symptom development of rice tungro disease [J].

Dai, SH ;

Zhang, ZH ;

Chen, SY ;

Beachy, RN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (02) :687-692

[7] Characterization of crosslinked starch materials with spectroscopic techniques [J].

Delval, F ;

Crini, G ;

Bertini, S ;

Morin-Crini, N ;

Badot, PM ;

Vebrel, JL ;

Torri, G .

JOURNAL OF APPLIED POLYMER SCIENCE, 2004, 93 (06) :2650-2663

[8] Mutational analysis of the pullulanase-type debranching enzyme of maize indicates multiple functions in starch metabolism [J].

Dinges, JR ;

Colleoni, C ;

James, MG ;

Myers, AM .

PLANT CELL, 2003, 15 (03) :666-680

[9] Determining the effects of microwave heating on the ordered structures of rice starch by NMR [J].

Fan, Daming ;

Ma, Wenrui ;

Wang, Liyun ;

Huang, Jianlian ;

Zhang, Fengmin ;

Zhao, Jianxin ;

Zhang, Hao ;

Chen, Wei .

CARBOHYDRATE POLYMERS, 2013, 92 (02) :1395-1401

[10] OPAQUE11 Is a Central Hub of the Regulatory Network for Maize Endosperm Development and Nutrient Metabolism [J].

Feng, Fan ;

Qi, Weiwei ;

Lv, Yuanda ;

Yan, Shumei ;

Xu, Liming ;

Yang, Awenyao ;

Yuan, Yue ;

Chen, Yihan ;

Zhao, Han ;

Song, Rentao .

PLANT CELL, 2018, 30 (02) :375-396

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