Anti-inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Induced Mastitis in Mice

被引：76

作者：

Jiang, Kangfeng ^{[1
]}

Ma, Xiaofei ^{[1
]}

Guo, Shuai ^{[1
]}

Zhang, Tao ^{[1
]}

Zhao, Gan ^{[1
]}

Wu, Haichong ^{[1
]}

Wang, Xiaoyan ^{[1
]}

Deng, Ganzhen ^{[1
]}

机构：

[1] Huazhong Agr Univ, Dept Clin Vet Med, Coll Vet Med, Wuhan, Hubei, Peoples R China

来源：

INFLAMMATION | 2018年 / 41卷 / 02期

基金：

中国国家自然科学基金;

关键词：

rosmarinic acid; mastitis; LPS; inflammation; TLR4; NF-kappa B; NF-KAPPA-B; ACUTE LUNG INJURY; INFLAMMATORY RESPONSES; INTRAMAMMARY INFUSION; SIGNALING PATHWAY; BOVINE MASTITIS; IN-VITRO; ACTIVATION; CELLS; LPS;

D O I：

10.1007/s10753-017-0700-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rosmarinic acid (RA), a type of food additives mainly extracted from rosemary, has been reported to possess anti-inflammatory activities in some previous studies. However, the effects of RA on lipopolysaccharide (LPS)-induced mastitis have not been reported. Here, we investigated the anti-inflammatory effects of RA on LPS-induced mastitis in mice and elucidated the potential mechanisms in mouse mammary epithelial cells (mMECs). RA treatment significantly ameliorated the mammary structural damage, and reduced the activity of myeloperoxidase. ELISA and qPCR results indicated that RA dose-dependently decreased the expression of TNF-alpha, IL-1 beta, and IL-6 both in tissues and mMECs. Furthermore, RA remarkably suppressed the protein levels of TLR4, MyD88, IRAK1, TRAF6, and p-IKK beta. In addition, RA was also found to inhibit LPS-induced NF-kappa B signaling pathway activation. These results suggest that RA effectively attenuates LPS-induced mastitis by inhibiting the TLR4/MyD88/NF-kappa B signaling pathway.

引用

页码：437 / 448

页数：12

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