Preparation and Characterization of Micro-patterned Hybrid Bilayer Membrane

Bio-mimetic membrane, hybrid bilayer membrane (HBM), was prepared on solid substrate using Self-Assemly and Vesicle Fusion (VF) methods. The HBM films were composed of self-assembled monolayer (SAM) of alkanthiol and phosphatidylcholine. Micro-patterned composite membranes of lipids were made on solid substrate with micro-patterned SAM by a micro-contact printing (mu CP) method. AFM and Kelvin probe force microscopy (KFM) observation was conducted under height and phase modes, and surface potential, respectively. The height imaging showed that the flat surface was formed by the mu CP method. Furthermore, the phase imaging and surface potential imaging revealed the micro-pattern formation. These results show that an artificial bilayer was successfully prepared on the surface without SAM, and lipid monolayer on the surface with SAM. These results were supported by the cyclic-voltammetry (CV) and surface potential.