Nerve growth factor induced neurite outgrowth from amphibian neuroepithelial precursor cells is prevented by dipeptides inhibiting ubiquitin-mediated proteolysis

被引：0

作者：

Maufroid, JP

Bradshaw, RA

Boilly, B

Hondermarck, H

机构：

[1] UNIV SCI & TECHNOL LILLE,UNITE DYNAM CELLULES EMBRYONNAIRES & CANCEREUSES,CTR BIOL CELLULAIRE,F-59655 VILLENEUVE DASCQ,FRANCE

[2] UNIV CALIF IRVINE,COLL MED,DEPT BIOL CHEM,IRVINE,CA 92717

来源：

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | 1996年 / 40卷 / 03期

关键词：

neuroepithelial precursor cells; growth factors; neurite outgrowth; ubiquitin mediated proteolysis; ubiquitin ligase (E3);

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effect of dipeptides known to inhibit the ubiquitin-mediated proteolysis has been examined on growth factor induced neurite outgrowth from amphibian neuroepithelial precursor cells in primary culture. Nerve growth factor (NGF) stimulated neuritogenesis from these cells but fibroblast growth factor 2 (FGF-2) only increased the number of melanophores. The neurite outgrowth induced by NGF was inhibited by the dipeptides blocking the ubiquitin mediated proteolysis (Leu-Ala and Leu-Gly) whereas the inactive control dipeptides (Ala-Leu and Ala-His) had no effect. This suggests that ubiquitin-mediated proteolysis involving the ubiquitin ligase E3 is necessary for growth factor induced neuronal differentiation during the development of the central nervous system.

引用

页码：609 / 611

页数：3

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