Fluorescent cyanine-guanidiniocarbonyl-pyrrole conjugate with pH-dependent DNA/RNA recognition and DPP III fluorescent labelling and inhibition properties

被引：6

作者：

Smidlehner, Tamara ^{[1
]}

Karacic, Zrinka ^{[1
]}

Tomic, Sanja ^{[1
]}

Schmuck, Carsten ^{[2
]}

Piantanida, Ivo ^{[1
]}

机构：

[1] Rudjer Boskovic Inst, Zagreb, Croatia

[2] Univ Duisburg Essen, Inst Organ Chem, Essen, Germany

来源：

MONATSHEFTE FUR CHEMIE | 2018年 / 149卷 / 07期

关键词：

DNA/RNA recognition; Circular dichroism; Fluorescence; DPP III enzyme binding; DPP III inhibition; NUCLEIC-ACIDS; BINDING; DNA; POLYNUCLEOTIDES; COMPLEXES; DYES;

D O I：

10.1007/s00706-018-2192-0

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here designed and prepared cyanine-guanidinocarbonyl-pyrrole conjugate (Cy-GCP), intrinsically non-fluorescent, revealed fluorescent switch-on recognition of various secondary structures of ds-DNA and ds-RNA. Moreover, at the same submicromolar concentrations, DNA/RNA recognition was observed by selective induced (I)CD pattern in the visible range. Preliminary results showed that Cy-GCP strongly interacted with DPP III enzyme, switching-on the fluorescence upon binding and inhibiting enzyme action with efficiency comparable to the best-known inhibitor tynorphin. [GRAPHICS] .

引用

页码：1307 / 1313

页数：7

共 24 条

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