Regeneration of dental pulp after pulpotomy by transplantation of CD31-/CD146- side population cells from a canine tooth

被引：98

作者：

Iohara, Koichiro ^{[1
]}

Zheng, Li ^{[1
]}

Ito, Masataka ^{[2
]}

Ishizaka, Ryo ^{[1
,4
]}

Nakamura, Hiroshi ^{[3
]}

Into, Takeshi ^{[1
]}

Matsushita, Kenji ^{[1
]}

Nakashima, Misako ^{[1
]}

机构：

[1] Natl Inst Longev Sci, Dept Oral Dis Res, Natl Ctr Geriatr & Gerontol, Aichi 4748522, Japan

[2] Natl Def Med Coll, Dept Dev Anat & Regenerat Med, Tokorozawa, Saitama 3598513, Japan

[3] Aichi Gakuin Univ, Sch Dent, Dept Endodontol, Aichi 4648651, Japan

[4] Aichi Gakuin Univ, Sch Dent, Dept Pediat Dent, Aichi 4648651, Japan

来源：

REGENERATIVE MEDICINE | 2009年 / 4卷 / 03期

关键词：

amputated pulp; CD31; dental pulp stem cells; dentinogenesis; pulp regeneration; side population cells; ENDOTHELIAL GROWTH-FACTOR; STEM-CELLS; TISSUE; INDUCTION; DIFFERENTIATION;

D O I：

10.2217/RME.09.5

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Aim: To achieve complete regeneration of dental pulp in vivo by stem/progenitor cells obtained from a fraction of side population (SP) cells from canine pulp. Materials & methods: A subfraction of SP cells, CD31(-)/CD146(-)SP cells, were isolated by flow cytometry from canine dental pulp. The efficiency of this subfraction of SP cells was evaluated in an experimental model of pulp injury in the dog. Results: The fractionated SP cells formed extensive networks of tube-like structures in vitro. Transplantation of the SP cells into an in vivo model of amputated pulp resulted in complete regeneration of pulp tissue with capillaries and neuronal cells within 14 days. Gene-expression studies demonstrated the expression of pro-angiogenic factors, implying trophic action on endothelial cells. Conclusions: This investigation demonstrates the potential utility of fractionated SP cells as a source of cells for total pulp regeneration complete with angiogenesis and vasculogenesis.

引用

页码：377 / 385

页数：9

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