Suppression of Bacterial Wilt of Tomato by Bioorganic Fertilizer Made from the Antibacterial Compound Producing Strain Bacillus amyloliquefaciens HR62

被引：40
作者：
Huang, Jianfeng
Wei, Zhong
Tan, Shiyong
Mei, Xinlan
Shen, Qirong
Xu, Yangchun [1 ]
机构：
[1] Nanjing Agr Univ, Natl Engn Res Ctr Organ Based Fertilizers, Nanjing 210095, Jiangsu, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2014年 / 62卷 / 44期
基金：
中国国家自然科学基金;
关键词：
bacterial wilt of tomato; antibacterial compounds; bioorganic fertilizer; biological control; Ralstonia solanacearum; FORTIFIED ORGANIC FERTILIZER; BIOLOGICAL-CONTROL; RHIZOCTONIA-SOLANI; FUSARIUM-WILT; SUBTILIS; BIOCONTROL; CUCUMBER; COLONIZATION; SOLANACEARUM; MACROLACTINS;
D O I：
10.1021/jf503136a
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Ralstonia solanacearum (Smith) is an important soil-borne pathogen worldwide. We investigated the effects of a new bioorganic fertilizer, BIO62, which was made from organic fertilizer and antagonist Bacillus amyloliquefaciens HR62, on the control of bacterial wilt of tomato in greenhouse condition. The results showed that the application of BIO62 significantly decreased disease incidence by 65% and strongly reduced R. solanacearum populations both in the rhizosphere soil (8.04 log cfu g(-1) dry soil) and crown sections (5.63 log cfu g(-1) fresh plant section) at 28 days after pathogen challenge. Antibacterial compounds produced by HR62 were purified by silica gel, Sephadex LH-20, and HPLC and then identified using HPLC/electrospray ionization mass spectrometry analysis. Macrolactin A and 7-O-malonyl macrolactin A (molecular weights of 402 and 488 Da, respectively), along with surfactin B (molecular weights of 994, 1008, 1022, and 1036 Da), were observed to inhibit the growth of R. solanacearum
引用
收藏
页码：10708 / 10716
页数：9
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