Cell wall structure of mushroom sclerotium (Pleurotus tuber-regium): Part 2. Fine structure of a novel alkali-soluble hyper-branched cell wall polysaccharide

被引:52
作者
Chen, Lei [1 ,2 ]
Zhang, Bo-Bo [3 ]
Chen, Jia-Lun [1 ,2 ]
Cheung, Peter C. K. [1 ,2 ]
机构
[1] Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China
[2] CUHK Shenzhen Res Inst, Shenzhen, Peoples R China
[3] Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China
关键词
Alkali-soluble; Cell wall polysaccharide; beta-glucan; Hyper-branched; Mushroom sclerotium; Pleurotus tuber-regium; ATOMIC-FORCE MICROSCOPY; PORIA-COCOS SCLEROTIUM; HYPERBRANCHED ACIDIC POLYSACCHARIDE; INDUCED CATIONIC-POLYMERIZATION; CHAIN CONFORMATION; FRUITING BODIES; WATER; DERIVATIVES; NANOPARTICLES; LENTINAN;
D O I
10.1016/j.foodhyd.2013.11.004
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The fine molecular structure of a novel alkali-soluble polysaccharide (SCA-I) from the mushroom sclerotial cell wall of Pleurotus tuber-regium was elucidated by 1-D and 2-D NMR spectroscopy as a hyper-branched glucan having a 64.9% degree of branching at 0-6 and consisting a main chain with -> 1)-Glcp-(4 -> linkages and short side chains having either -> 1)-Glcp-(3 -> or -> 1)-Glcp-(6 -> linkages. This partial structure of SCA-I was consistent with that obtained from methylation analysis. The molecular conformation of SDS-treated SCA-I was visualized for the first time by TEM and AFM. Microscopic images confirmed the highly branched structure of SCA-I as well as its multiple chain conformation when its compact structure was opened up by SDS treatment. This unique hyper-branched polysaccharide has the potential application in the design of carbohydrate-based nano-particles and drug delivery systems. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:48 / 55
页数:8
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