SCREENING OF ASPERGILLUS NIGER AND A-FLAVUS STRAINS FOR EXTRA CELLULAR ALPHA-AMYLASE ACTIVITY

被引：0

作者：

Shafique, Sobiya ^{[1
]}

Bajwa, Rukhsana ^{[1
]}

Shafique, Shazia ^{[1
]}

机构：

[1] Univ Punjab, Inst Mycol & Plant Pathol, Lahore, Pakistan

来源：

PAKISTAN JOURNAL OF BOTANY | 2009年 / 41卷 / 02期

关键词：

FERMENTATION;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Five fungal strains of each of the two filamentous fungi viz., Aspergillus niger Van Tieghem and A. flavus Link ex Gray, indigenous to Pakistan, were screened for their alpha-amylase activity. Different selected strains were grown on two growth media viz., Potato Dextrose Agar (PDA) and Enzyme Production Medium (EPM), at three pH levels viz., 4.5, 5.5 and 6.5, in all the possible combinations. Performance of various strains was recorded in terms of hydrolyzing zone formation. Generally test strains exhibited their best performance on EPM at pH 4.5. On the basis of their best performance on solid media, strain 74 and strain 198 of A. niger and strain 209 and strain 231 of A. flavus were selected for periodic evaluation of their alpha-amylase activity in liquid medium, using shake flask technique. All the test strains exhibited their maximum alpha-amylase activity after 48 h

引用

页码：897 / 905

页数：9

共 22 条

[1] PRODUCTION OF THE RAW-STARCH DIGESTING AMYLASE OF ASPERGILLUS SP K-27
ABE, J
BERGMANN, FW
OBATA, K
HIZUKURI, S
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1988, 27 (5-6) : 447 - 450
[2] Akpan I., 1999, Tropical Science, V39, P77
[3] COMPARATIVE-STUDY OF AMYLOLYTIC ENZYMES PRODUCTION BY ASPERGILLUS-NIGER IN LIQUID AND SOLID-STATE CULTIVATION
ALAZARD, D
RAIMBAULT, M
[J]. EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1981, 12 (02): : 113 - 117
[4] Extracellular enzymatic activity profiles in yeast and yeast-like strains isolated from tropical environments
Buzzini, P
Martini, A
[J]. JOURNAL OF APPLIED MICROBIOLOGY, 2002, 93 (06) : 1020 - 1025
[5] Elander R.P, 1982, OVERPRODUCTION MICRO, P353
[6] Fadel M., 2000, Egyptian Journal of Microbiology, V35, P487
[7] Haq I., 2002, Pakistan Journal of Biological Sciences, V2, P73, DOI [DOI 10.3923/JBS.2002.73.75, 10.3923/jbs.2002.73.75]
[8] HAQ IR, 2002, BIOTECHNOLOGY, V1, P61
[9] Influence of culture media and environmental factors on mycelial growth and pycnidial production of Sphaeropsis pyriputrescens
Kim, YK
Xiao, CL
Rogers, JD
[J]. MYCOLOGIA, 2005, 97 (01) : 25 - 32
[10] Mikiashvili N., 2004, International Journal of Medicinal Mushrooms, V6, P63, DOI [10.1615/IntJMedMushr.v6.i1.70, DOI 10.1615/IntJMedMushr.v6.i1.70]

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