Isolation, purification and antibacterial effects of fusaproliferin produced by Fusarium subglutinans in submerged culture

被引：16

作者：

Meca, G. ^{[1
]}

Sospedra, I. ^{[1
]}

Soriano, J. M. ^{[1
]}

Ritieni, A. ^{[2
]}

Valero, M. A. ^{[3
]}

Manes, J. ^{[1
]}

机构：

[1] Univ Valencia, Fac Pharm, Lab Food Chem & Toxicol, E-46100 Burjassot, Spain

[2] Univ Naples Federico II, Dept Food Sci, I-80055 Naples, Italy

[3] Univ Valencia, Fac Pharm, Dept Parasitol, E-46100 Burjassot, Spain

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2009年 / 47卷 / 10期

关键词：

Fusaproliferin; Fusarium subglutinans; Semipreparative column; High-performance liquid chromatography; Mass spectrometry; MYCOTOXINS FUSAPROLIFERIN; ARTEMIA-SALINA; BEAUVERICIN; MONILIFORMIN; ENNIATINS; TOXICITY; TEMPERATURE; SUBSTRATE; GROWTH; B-1;

D O I：

10.1016/j.fct.2009.07.014

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To evaluate the fusaproliferin (FUS) production, Fusarium subglutinans ITEM 2404 was grown in a liquid medium of potato being this mycotoxin purified by high-performance liquid chromatography (HPLC) with a C18 semipreparative column using a mobile phase of acetonitrile/H2O using gradient conditions. The purity of the fusaproliferin was verified by analytical HPLC, ultraviolet absorbance measurements, LC/MS-MS, H-1 NMR spectroscopy. The isolated FUS was shown to be free of impurities and can be used as a standard for routine analysis. The pure fusaproliferin was utilized to study the biological activity on Escherichia coli and Staphylococcus aureus. This study demostred that FUS not showed significant antimicrobial activity against these microorganisms. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2539 / 2543

页数：5

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