Cells, drugs and NMR

被引：50

作者：

Siegal, Gregg ^{[1
]}

Selenko, Philipp ^{[2
]}

机构：

[1] ZoBio BV, BioPartner 2 Bldg,JH Oortweg 19, NL-2333 Leiden, Netherlands

[2] Weizmann Inst Sci, Dept Biol Regulat, 234 Herzl St, IL-761000 Rehovot, Israel

来源：

JOURNAL OF MAGNETIC RESONANCE | 2019年 / 306卷

基金：

欧洲研究理事会;

关键词：

In-cell NMR; In vivo NMR; On-cell NMR; Whole-cell NMR; Metabolomics; Solid-state NMR; Dynamic nuclear polarization; SOLID-STATE NMR; INTRINSICALLY DISORDERED PROTEINS; MAGNETIC-RESONANCE-SPECTROSCOPY; IN-VIVO; ESCHERICHIA-COLI; LIVING CELLS; STAPHYLOCOCCUS-AUREUS; MULTIDIMENSIONAL NMR; QUINARY INTERACTIONS; MEMBRANE-PROTEINS;

D O I：

10.1016/j.jmr.2019.07.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear magnetic resonance (NMR) spectroscopy is a versatile tool for investigating cellular structures and their compositions. While in vivo and whole-cell NMR have a long tradition in cell-based approaches, high-resolution in-cell NMR spectroscopy is a new addition to these methods. In recent years, technological advancements in multiple areas provided converging benefits for cellular MR applications, especially in terms of robustness, reproducibility and physiological relevance. Here, we review the use of cellular NMR methods for drug discovery purposes in academia and industry. Specifically, we discuss how developments in NMR technologies such as miniaturized bioreactors and flow-probe perfusion systems have helped to consolidate NMR's role in cell-based drug discovery efforts. (C) 2019 The Author(s). Published by Elsevier Inc.

引用

页码：202 / 212

页数：11

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