Real-time optoacoustic brain microscopy with hybrid optical and acoustic resolution

被引：74

作者：

Estrada, Hector ^{[1
]}

Turner, Jake ^{[1
,2
]}

Kneipp, Moritz ^{[1
,3
]}

Razansky, Daniel ^{[1
,3
]}

机构：

[1] Helmholtz Ctr Munich, IBMI, D-85764 Neuherberg, Germany

[2] Tech Univ Munich, Fac Elect Engn & Informat Technol, D-80333 Munich, Germany

[3] Tech Univ Munich, Fac Med, D-81675 Munich, Germany

来源：

LASER PHYSICS LETTERS | 2014年 / 11卷 / 04期

基金：

欧洲研究理事会;

关键词：

optoacoustic imaging; optoacoustic microscopy; in vivo imaging; transcranial imaging; hybrid focusing; IN-VIVO; PHOTOACOUSTIC MICROSCOPY;

D O I：

10.1088/1612-2011/11/4/045601

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Conventional optoacoustic microscopy operates in two distinct modes of optical resolution, for visualization of superficial tissue layers, or acoustic resolution, intended for deep imaging in scattering tissues. Here we introduce a new microscope design with hybrid optical and acoustic resolution, which provides a smooth transition from optical resolution in superficial microscopic imaging to ultrasonic resolution when imaging at greater depths within intensely scattering tissue layers. Experimental validation of the new hybrid optoacoustic microscopy method was performed in phantoms and by means of transcranial mouse brain imaging in vivo.

引用

页数：4

共 17 条

[1] Model-based optoacoustic imaging using focused detector scanning [J].

Caballero, Miguel Angel Araque ;

Rosenthal, Amir ;

Gateau, Jerome ;

Razansky, Daniel ;

Ntziachristos, Vasilis .

OPTICS LETTERS, 2012, 37 (19) :4080-4082

[2] Blind-deconvolution optical-resolution photoacoustic microscopy in vivo [J].

Chen, Jianhua ;

Lin, Riqiang ;

Wang, Huina ;

Meng, Jing ;

Zheng, Hairong ;

Song, Liang .

OPTICS EXPRESS, 2013, 21 (06) :7316-7327

[3] A fiber-optic system for dual-modality photoacoustic microscopy and confocal fluorescence microscopy using miniature components [J].

Chen, Sung-Liang ;

Xie, Zhixing ;

Guo, L. Jay ;

Wang, Xueding .

PHOTOACOUSTICS, 2013, 1 (02) :30-35

[4]

Judkewitz B, 2013, NAT PHOTONICS, V7, P300, DOI [10.1038/NPHOTON.2013.31, 10.1038/nphoton.2013.31]

[5] High-numerical-aperture-based virtual point detectors for photoacoustic tomography [J].

Li, Changhui ;

Wang, Lihong V. .

APPLIED PHYSICS LETTERS, 2008, 93 (03)

[6] Transcranial imaging of functional cerebral hemodynamic changes in single blood vessels using in vivo photoacoustic microscopy [J].

Liao, Lun-De ;

Lin, Chin-Teng ;

Shih, Yen-Yu I. ;

Duong, Timothy Q. ;

Lai, Hsin-Yi ;

Wang, Po-Hsun ;

Wu, Robby ;

Tsang, Siny ;

Chang, Jyh-Yeong ;

Li, Meng-Lin ;

Chen, You-Yin .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2012, 32 (06) :938-951

[7] Fast scanning coaxial optoacoustic microscopy [J].

Ma, Rui ;

Soentges, Sebastian ;

Shoham, Shy ;

Ntziachristos, Vasilis ;

Razansky, Daniel .

BIOMEDICAL OPTICS EXPRESS, 2012, 3 (07) :1724-1731

[8]

MAEV R.G., 2008, ACOUSTIC MICROSCOPY

[9]

Maslov K, 2005, OPT LETT, V30, P625, DOI 10.1364/OL.30.6.000625

[10] Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries [J].

Maslov, Konstantin ;

Zhang, Hao F. ;

Hu, Song ;

Wang, Lihong V. .

OPTICS LETTERS, 2008, 33 (09) :929-931

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