Clarifying the Translational Pausing Landscape in Bacteria by Ribosome Profiling

被引：128

作者：

Mohammad, Fuad ^{[1
]}

Woolstenhulme, Christopher J. ^{[1
]}

Green, Rachel ^{[1
,2
]}

Buskirk, Allen R. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, 725 North Wolfe St, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, 725 North Wolfe St, Baltimore, MD 21205 USA

来源：

CELL REPORTS | 2016年 / 14卷 / 04期

关键词：

MESSENGER-RNA TRANSLOCATION; SHINE-DALGARNO SEQUENCE; PROTEIN-SYNTHESIS; ESCHERICHIA-COLI; IN-VIVO; EF-P; ELONGATION; INITIATION; CODON; REVEALS;

D O I：

10.1016/j.celrep.2015.12.073

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The rate of protein synthesis varies according to the mRNA sequence in ways that affect gene expression. Global analysis of translational pausing is now possible with ribosome profiling. Here, we revisit an earlier report that Shine-Dalgarno sequences are the major determinant of translational pausing in bacteria. Using refinements in the profiling method as well as biochemical assays, we find that SD motifs have little (if any) effect on elongation rates. We argue that earlier evidence of pausing arose from two factors. First, in previous analyses, pauses at Gly codons were difficult to distinguish from pauses at SD motifs. Second, and more importantly, the initial study preferentially isolated long ribosome-protected mRNA fragments that are enriched in SD motifs. These findings clarify the landscape of translational pausing in bacteria as observed by ribosome profiling.

引用

页码：686 / 694

页数：9

共 37 条

[1] The conserved GTPase LepA contributes mainly to translation initiation in Escherichia coli [J].