Estimating telomere length from whole genome sequence data

被引：123
作者：
Ding, Zhihao [1 ]
Mangino, Massimo [2 ]
Aviv, Abraham [3 ]
Spector, Tim [2 ]
Durbin, Richard [1 ]
机构：
[1] Wellcome Trust Sanger Inst, Hinxton CB10 1SA, Cambs, England
[2] Kings Coll London, Dept Twin Res & Genet Epidemiol, London WC2R 2LS, England
[3] Rutgers State Univ, Ctr Human Dev & Aging, New Jersey Med Sch, Newark, NJ 07103 USA
来源：
NUCLEIC ACIDS RESEARCH | 2014年 / 42卷 / 09期
基金：
美国国家卫生研究院; 英国惠康基金;
关键词：
DNA-CONTENT;
D O I：
10.1093/nar/gku181
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.
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页数：4
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