Enzymatic Electrochemical Biosensor Based on Multiwall Carbon Nanotubes and Cerium Dioxide Nanoparticles for Rutin Detection

被引：18

作者：

Benjamin, Stephen Rathinaraj ^{[1
,2
]}

Vilela, Ramon Silva ^{[1
,3
]}

de Camargo, Henrique Santiago ^{[1
]}

Florindo Guedes, Maria Izabel ^{[2
]}

Fernandes, Katia Flavia ^{[3
]}

Colmati, Flavio ^{[1
]}

机构：

[1] Univ Fed Goias, IQ UFG, Inst Quim, Lab Bioelectrocatalysis & Fuel Cells LABEL FC, Av Esperanca S-N, BR-74690900 Goiania, Go, Brazil

[2] Univ Estadual Ceara UECE, Grp Inovacao Biotecnol Saude, LBBM, Av Paranjana,1700 Campus Itaperi, BR-60714903 Fortaleza, Ceara, Brazil

[3] Univ Fed Goias ICB UFG, Lab Quim Polimeros, Inst Ciencias Biol, Dept Ciencias Fisiol, Av Esperanca S-N, BR-74690900 Goiania, Go, Brazil

来源：

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2018年 / 13卷 / 01期

关键词：

Multiwall carbon nanotubes; Cerium dioxide nanoparticles; Crude extract; Rutin; POx; DIRECT ELECTRON-TRANSFER; HORSERADISH-PEROXIDASE; PHENOLIC-COMPOUNDS; CRUDE EXTRACT; IONIC LIQUID; LACCASE; NANOMATERIALS; CYCLODEXTRIN; GLUCOSE;

D O I：

10.20964/2018.01.51

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

In this study, a simple and sensitive enzymatic electrochemical biosensor was developed to detect rutin by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and square wave voltammetry (SWV) using a carbon paste electrode modified with a multiwall carbon nanotube (MWCNT), cerium oxide nanoparticle (CeO2), and crude extract source of peroxidase enzyme (POx) composite. The electrochemical parameters and experimental conditions were optimized and evaluated. The enzymatic electrochemical biosensor (CeO2/POx/MWCNTs/CPE) showed excellent electrocatalytic activity towards the detection of rutin. The surface physical characteristics of the modified electrode were studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). This biosensor demonstrated selectivity, stability, and reproducibility, which was further applied to detect rutin in medicine tablets and capsules with recoveries in the range of 97-102%.

引用

页码：563 / 586

页数：24

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