Isolation of the new minor constituents dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography

被引：18

作者：

Skalicka-Wozniak, Krystyna ^{[1
]}

Mroczek, Tomasz ^{[1
]}

Garrard, Ian ^{[2
]}

Glowniak, Kazimierz ^{[1
]}

机构：

[1] Med Univ Lublin, Dept Pharmacognosy, Med Plant Lab, PL-20093 Lublin, Poland

[2] Brunel Univ, Adv Bioproc Ctr, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 30期

关键词：

High-speed counter-current chromatography; Peucedanum alsaticum; Coumarin; Pyranochromone; Preparative chromatography; TRADITIONAL MEDICINAL HERB; PREPARATIVE ISOLATION; PURIFICATION; COUMARINS; COLUMN; ROOT;

D O I：

10.1016/j.chroma.2009.05.077

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for isolation of two new minor compounds - alsaticol and alsaticocoumarin A. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:1:1:1) was developed. Compounds were obtained from the dichloromethane extract of Peucedanum alsaticum fruits and their identification was performed with NMR and MS methods. Optimized HSCCC offers a rapid method of obtaining new natural compounds. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：5669 / 5675

页数：7

共 27 条

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