Effect of different sample volumes on the DNA extraction of Aspergillus fumigatus from whole blood

被引：8

作者：

Schulz, B. ^{[1
]}

Weber, K. ^{[1
]}

Radecke, C. ^{[1
]}

Scheer, C. ^{[1
]}

Ruhnke, M. ^{[1
]}

机构：

[1] Charite, Med Klin Schwerpunkt Onkol & Hamatol, Dept Med, Div Oncol & Haematol, D-10117 Berlin, Germany

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2009年 / 15卷 / 07期

关键词：

Aspergillus fumigatus; DNA extraction; PCR (polymerase chain reaction); sample volume; whole blood; POLYMERASE-CHAIN-REACTION; BRONCHOALVEOLAR LAVAGE SAMPLES; IMMUNOCOMPROMISED PATIENTS; CANDIDA-ALBICANS; FUNGAL PATHOGENS; PCR;

D O I：

10.1111/j.1469-0691.2009.02797.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

P>Five methods were compared, using conventional PCR, for the isolation of DNA from Aspergillus fumigatus conidia from 1-3-mL samples of whole blood. A lower detection threshold of Aspergillus conidia was achieved using 3-mL rather than 1-mL samples with three of five methods tested.

引用

页码：687 / 688

页数：2

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