Juvenile hormone regulates Aedes aegypti Kruppel homolog 1 through a conserved E box motif

被引：74

作者：

Cui, Yingjun ^{[1
]}

Sui, Yipeng ^{[1
]}

Xu, Jingjing ^{[1
]}

Zhu, Fang ^{[1
]}

Palli, Subba Reddy ^{[1
]}

机构：

[1] Univ Kentucky, Dept Entomol, Coll Agr, Lexington, KY 40546 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2014年 / 52卷

基金：

美国国家卫生研究院;

关键词：

Juvenile hormone; Methoprene-tolerant; Kruppel homolog 1; bHLH | JH response element; METHOPRENE-TOLERANT; DROSOPHILA-MELANOGASTER; GENE-EXPRESSION; METAMORPHOSIS; PROTEINS; BINDING; KRUPPEL-HOMOLOG-1; HETERODIMER; RECEPTOR; FAMILY;

D O I：

10.1016/j.ibmb.2014.05.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Juvenile hormone (JH) plays important roles in regulation of many physiological processes including development, reproduction and metabolism in insects. However, the molecular mechanisms of JH signaling pathway are not completely understood. To elucidate the molecular mechanisms of JH regulation of Kruppel homolog 1 gene (Kr-h1) in Aedes aegypti, we employed JH-sensitive Aag-2 cells developed from the embryos of this insect. In Aag-2 cells, AaKr-h1 gene is induced by nanomolar concentration of JH III, its expression peaked at 1.5 h after treatment with JH III. RNAi studies showed that JH induction of this gene requires the presence of Ae. aegypti methoprene-tolerant (AaMet). A conserved 13 nucleotide JH response element (JHRE, TGCCTCCACGTGC) containing canonical E box motif (underlined) identified in the promoter of AaKr-h1 is required for JH induction of this gene. Critical nucleotides in the JHRE required for JH action were identified by employing mutagenesis and reporter assays. Reporter assays also showed that basic helix loop helix (bHLH) domain of AaMet is required for JH induction of AaKr-h1. 5' rapid amplification of cDNA ends method identified two isoforms of AaKr-h1, AaKr-h1 alpha and AaKr-h1 beta, the expression of both isoforms is induced by JH III, but AaKr-h1 alpha is the predominant isoform in both Aag-2 cells and Ae. aegypti larvae. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：23 / 32

页数：10

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