Fluorescent DNA Probes Based on Quantum Dots and Core/Shell Quantum Dots

被引：0

作者：

Song, Mengmeng ^{[1
]}

Guo, Wenjuan ^{[1
]}

Dai, Zhao ^{[1
]}

Qiu, Kaili ^{[1
]}

Wei, Junfu ^{[1
]}

机构：

[1] Tianjin Polytech Univ, Sch Environm & Chem Engn, State Key Lab Hollow Fiber Membrane Mat & Proc, Tianjin, Peoples R China

来源：

ADVANCED MATERIALS DESIGN AND MECHANICS II | 2013年 / 372卷

关键词：

DNA probe; Au Nanoparticles; Quantum dots; Core/Shell; FRET; RESONANCE ENERGY-TRANSFER; NANOPARTICLES; PROTEIN;

D O I：

10.4028/www.scientific.net/AMM.372.115

中图分类号：

TU [建筑科学];

学科分类号：

0813 ;

摘要：

Fluorescent DNA probes based on the fluorescence resonance energy transfer (FRET) were presented in this paper when AuNPs were as the energy acceptors and CdTe quantum dots and CdTe/SiO2 core/shell nanoparticles were as the energy acceptors, respectively. The DNA probes were prepared when energy donors and acceptors were conjugated with two single-stranded complementary oligonucleotides and hybridized with each other and the fluorescent intensity of probes could be decreased. The quenching efficiency of DNA probe was about 67 % when CdTe QDs were as the energy donors, while that of DNA probe was about 75 % when CdTe/SiO2 fluorescent core/shell nanoparticles were as the energy acceptors, which indicated that CdTe/SiO2 core/shell nanoparticles were suitable donors compared with CdTe QDs in DNA probe field.

引用

页码：115 / 118

页数：4

共 10 条

[1] Luminescent CdS quantum dots as selective ion probes [J].