Proteomic characterization of seeds from yellow lupin (Lupinus luteus L.)

被引：18

作者：

Ogura, Takahiro ^{[1
]}

Ogihara, Jun ^{[2
]}

Sunairi, Michio ^{[3
]}

Takeishi, Hidetaka ^{[3
]}

Aizawa, Tomoko ^{[3
]}

Olivos-Trujillo, Marcos R. ^{[1
]}

Maureira-Butler, Ivan J. ^{[1
]}

Salvo-Garrido, Haroldo E. ^{[1
,4
]}

机构：

[1] CGNA, Genom & Bioinformat Unit, Agriaquaculture Nutr Genom Ctr, Temuco 4791057, Chile

[2] Nihon Univ, Dept Chem & Life Sci, Coll Bioresource Sci, Fujisawa, Kanagawa, Japan

[3] Nihon Univ, Dept Appl Biol Sci, Coll Bioresource Sci, Fujisawa, Kanagawa, Japan

[4] Inst Agr Res INIA, Carillanca, Temuco, Chile

来源：

PROTEOMICS | 2014年 / 14卷 / 12期

关键词：

2DE; Legume seed; Lupinus luteus L; Nano-LC-MS/MS; Plant proteomics; PROTEINS; ALBUS; PURIFICATION;

D O I：

10.1002/pmic.201300511

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).

引用

页码：1543 / 1546

页数：4

共 50 条

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