Mutation detection of the human glucocorticoid receptor alpha gene area coding for the hormone-binding domain by denaturing gradient gel electrophoresis

被引：2

作者：

Tsolakidou, AF ^{[1
]}

Coulocheri, SA ^{[1
]}

Sekeris, CE ^{[1
]}

Moutsatsou, P ^{[1
]}

机构：

[1] Univ Athens, Sch Med, Biol Chem Lab, GR-11527 Athens, Greece

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2002年 / 51卷 / 03期

关键词：

DGGE; glucocorticoid receptor; mutation detection;

D O I：

10.1016/S0165-022X(02)00012-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mutations in the hormone-binding domain of the human glucocorticoid receptor alpha (hGRalpha) gene have been detected in a variety of glucocorticoid resistance syndromes. Using the denaturing gradient gel electrophoresis technique, we developed a sensitive method for the detection of alterations in the gene area coding for the whole hormone-binding domain and part of the DNA-binding domain of the hGRalpha. This method can be applied for screening of glucocorticoid receptor gene alterations in glucocorticoid-dependent diseases. (C) 2002 Elsevier Science B.V. All tights reserved.

引用

页码：233 / 241

页数：9

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