Vitamin C enhances anticancer activity in methotrexate-treated Hep3B hepatocellular carcinoma cells

被引：32

作者：

Yiang, Giou-Teng ^{[1
,2
]}

Chou, Pei-Lun ^{[3
,4
]}

Hung, Yu-Ting ^{[5
]}

Chen, Jen-Ni ^{[6
]}

Chang, Wei-Jung ^{[7
,8
]}

Yu, Yung-Luen ^{[7
,8
,9
]}

Wei, Chyou-Wei ^{[5
]}

机构：

[1] Buddhist Tzu Chi Med Fdn, Taipei Tzu Chi Hosp, Dept Emergency Med, New Taipei 231, Taiwan

[2] Tzu Chi Univ, Sch Med, Dept Emergency Med, Hualien 970, Taiwan

[3] St Marys Hosp Luodong, Dept Internal Med, Div Allergy Immunol Rheumatol, Yilan 265, Taiwan

[4] Taipei Med Univ, Coll Med, Sch Med, Dept Internal Med, Taipei 110, Taiwan

[5] Hungkuang Univ, Master Program Biomed Nutr, Dept Nutr, Taichung 433, Taiwan

[6] Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, Taichung 402, Taiwan

[7] China Med Univ, Grad Inst Canc Biol, Taichung 404, Taiwan

[8] China Med Univ, Ctr Mol Med, Taichung 404, Taiwan

[9] Asia Univ, Dept Biotechnol, Taichung 413, Taiwan

来源：

ONCOLOGY REPORTS | 2014年 / 32卷 / 03期

关键词：

methotrexate; vitamin C; apoptosis; hepatocellular carcinoma; GREEN TEA EXTRACT; RANA-CATESBEIANA RIBONUCLEASE; INDUCED OXIDATIVE STRESS; BREAST-CANCER CELLS; ASCORBIC-ACID; N-ACETYLCYSTEINE; DOWN-REGULATION; INDUCED APOPTOSIS; HEPATOMA-CELLS; COMBINATION THERAPY;

D O I：

10.3892/or.2014.3289

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Methotrexate (MTX) has been widely used for rheumatoid arthritis therapy for a long time. MTX is also used as an anticancer drug for various tumors. However, many studies have shown that high-dose MTX treatment for cancer therapy may cause liver and renal damage. Alhough the mechanisms involved in MTX-induced liver and renal damage require further research, many studies have indicated that MTX-induced cytotoxicity is associated with increases in oxidative stress and caspase activation. In order to reduce MTX-induced side-effects and increase anticancer efficiency, currently, combination treatments of low-dose MTX and other anticancer drugs are considered and applied for various tumor treatments. The present study showed that MTX induces increases in H2O2 levels and caspase-9/-3 activation leading to cell death in hepatocellular carcinoma Hep3B cells. Importantly, this study is the first to demonstrate that vitamin C can efficiently aid low-dose MTX in inducing cell death in Hep3B cells. Therefore, the present study provides a possible powerful therapeutic method for tumors using a combined treatment of vitamin C and low-dose MTX.

引用

页码：1057 / 1063

页数：7

共 72 条

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