An Activatable Fluorescent γ-Polyglutamic Acid Complex for Sentinel Lymph Node Imaging

被引：0

作者：

Hagimori, Masayori ^{[1
,2
]}

Hatabe, Eri ^{[1
]}

Sano, Kohei ^{[1
,3
,4
]}

Miyazaki, Hirotaka ^{[1
]}

Sasaki, Hitoshi ^{[5
]}

Saji, Hideo

Mukai, Takahiro ^{[1
]}

机构：

[1] Kobe Pharmaceut Univ, Dept Biophys Chem, Higashinada Ku, 4-19-1 Motoyama Kitamachi, Kobe, Hyogo 6588558, Japan

[2] Nagasaki Univ, Grad Sch Biomed Sci, Dept Pharmaceut Sci, 1-7-1 Sakamoto, Nagasaki 8528501, Japan

[3] Kyoto Univ Hosp, Radioisotopes Res Lab, Sakyo Ku, 54 Shogoin Kawahara Cho, Kyoto 6068507, Japan

[4] Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Pathofunct Bioanal, 46-29 Yoshida Shimoadachi Cho, Kyoto 6068501, Japan

[5] Nagasaki Univ, Hosp Med & Dent, Hosp Pharm, 1-7-1 Sakamoto, Nagasaki 8528501, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2017年 / 40卷 / 03期

关键词：

fluorescence-based optical imaging; sentinel lymph node; activatable; self-assembly; nanoparticle; SURFACE-CHARGE; GOLD NANOPARTICLES; ICG FLUORESCENCE; CANCER; CELLS; CYTOTOXICITY; CARCINOMA; APOPTOSIS; BIOPSY; PROBES;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Sentinel lymph nodes (SLN) are the first lymph nodes (LN) where cancer cells metastasize from the primary tumor. We designed fluorophore-quencher-based activatable nanoparticles for SLN imaging. We selected TAMRA as a fluorophore and BHQ2 or QSY7 as a quencher. Ternary anionic complexes were constructed with generation 4th polyamidoamine dendrimer (G4) modified with TAMRA and p-SCN-Bn-DTPA (DTPA), polyethyleneimine (PEI) modified with BHQ2 or QSY7, and gamma-polyglutamic acid (gamma-PGA) by the electrostatic self-assembly system. TAMRA-G4-DTPA/PEI-BHQ2/gamma-PGA and TAMRA-G4-DTPA/PEI-QSY7/gamma-PGA complexes had a particle size of about 40nm and a zeta -potential of 50 mV, and showed fluorescence resonance energy transfer (FRET) quenching. Fluorescence microscopy studies demonstrated that TAMRA-G4-DTPA/PEI-QSY7/gamma-PGA complex produced intracellular fluorescent signals in the lysosome. During in vivo fluorescent imaging, TAMRA-G4-DTPA/PEI-QSY7/gamma-PGA complex enabled the detection of mouse popliteal LN. The fluorophore-quencher conjugated gamma-PGA complex based on FRET quenching would be useful for fluorescence-based optical imaging of SLN.

引用

页码：297 / 302

页数：6

共 37 条

[1]

Bagby Taryn R, 2012, Pharmaceutics, V4, P276, DOI 10.3390/pharmaceutics4020276

[2] Role of surface charge and oxidative stress in cytotoxicity of organic monolayer-coated silicon nanoparticles towards macrophage NR8383 cells [J].