Adipose-derived stem cells from the breast

被引:1
作者
Yang, Jie [1 ]
Xiong, Lingyun [1 ,2 ]
Wang, Rongrong [1 ]
Sun, Jiaming [1 ]
Hirche, Christoph [2 ]
机构
[1] Huazhong Univ Sci & Technol, Union Hosp, Dept Plast & Reconstruct Surg, Wuhan 430074, Hubei, Peoples R China
[2] Heidelberg Univ, BG Trauma Ctr Ludwigshafen, Dept Hand Plast & Reconstruct Surg, Ludwigshafen, Germany
来源
JOURNAL OF RESEARCH IN MEDICAL SCIENCES | 2014年 / 19卷 / 02期
基金
中国国家自然科学基金;
关键词
Adipose-derived stem cells; breast; mammary gland; STROMAL CELLS; IN-VITRO; TISSUE; BONE; FAT;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: The adipose tissue is deemed as an ideal source of adipose-derived stem cells (ADSCs). Previous studies have reported that ADSCs can be isolated from several organs and locations; however, slight attention has been paid to the breast. We would like to report our experiences in isolating breast ADSCs (bADSCs). Materials and Methods: Adipose tissues were harvested from the breasts of seven hypertrophic breast patients. Collagenase I was used to isolate the primary ADSCs. Surface markers were analyzed by flow cytometry. Cellular morphologies were observed. Proliferations of different passages were compared. Viabilities after the cryopreservation were evaluated. Adipogenic and osteogenic differentiation was induced. Results: Primary cultured cells showed morphologies similar to fibroblasts, and expressed surface markers including CD13, CD44, CD90, and CD105. There was no statistical difference of proliferation between different passages (P > 0.05) and between with and without cryopreservation (P > 0.05). Additionally, isolated cells were differentiated into adipocytes and osteoblasts. Conclusion: bADSCs may represent an alternative candidate for tissue engineering. Further studies are needed to obtain more comprehensive understanding on bADSCs.
引用
收藏
页码:112 / 116
页数:5
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