Characterization of Novel Dipeptidyl Peptidase-IV Inhibitory Peptides from Soft-Shelled Turtle Yolk Hydrolysate Using Orthogonal Bioassay-Guided Fractionations Coupled with In Vitro and In Silico Study

被引：27
作者：
Nong, Nhung Thi Phuong [1 ,2 ]
Chen, Yu-Kuo [3 ]
Shih, Wen-Ling [4 ]
Hsu, Jue-Liang [4 ,5 ,6 ]
机构：
[1] Natl Pingtung Univ Sci & Technol, Dept Trop Agr & Int Cooperat, Pingtung 91201, Taiwan
[2] Thainguyen Univ Agr & Forestry, Dept Basic Sci, Thai Nguyen 250000, Vietnam
[3] Natl Pingtung Univ Sci & Technol, Dept Food Sci, Pingtung 91201, Taiwan
[4] Natl Pingtung Univ Sci & Technol, Dept Biol Sci & Technol, Pingtung 91201, Taiwan
[5] Natl Pingtung Univ Sci & Technol, Int Masters Degree Program Food Sci, Pingtung 91201, Taiwan
[6] Natl Pingtung Univ Sci & Technol, Res Ctr Anim Biol, Pingtung 91201, Taiwan
来源：
PHARMACEUTICALS | 2020年 / 13卷 / 10期
关键词：
DPP-IV inhibitory peptides; soft-shelled turtle yolk protein; bioassay-guided fractionation; LC-MS/MS; in silico analysis; PROTEIN-DERIVED PEPTIDES; DPP-IV; BIOACTIVE PEPTIDES; IDENTIFICATION; ANTIOXIDANT; CHALLENGES; POWDER;
D O I：
10.3390/ph13100308
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Five novel peptides (LPLF, WLQL, LPSW, VPGLAL, and LVGLPL) bearing dipeptidyl peptidase IV (DPP-IV) inhibitory activities were identified from the gastrointestinal enzymatic hydrolysate of soft-shelled turtle yolk (SSTY) proteins. Peptides were isolated separately using reversed-phase (RP) chromatography in parallel with off-line strong cation exchange (SCX) chromatography followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to determine sequences. Among these peptides, LPSW showed the highest DPP-IV inhibitory activity with an IC50 value of 269.7 +/- 15.91 mu M. The results of the pre-incubation experiment and the kinetic study of these peptides indicated that WLQL is a true inhibitor and its inhibition toward DPP-IV is of an uncompetitive model, while LPLF, LPSW, and VPGLAL are real-substrates and competitive inhibitors against DPP-IV. The DPP-IV inhibitory peptides derived from SSTY hydrolysate in study are promising in the management of hyperglycemia in Type 2 diabetes.
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页码：1 / 21
页数：21
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