Class 1 integrons as predominant carriers in Escherichia coli isolates from waterfowls in Hainan, China

被引：22

作者：

Zhang, Shaqiu ^{[1
,2
,3
]}

Yang, Hong ^{[1
,2
,3
]}

Rehman, Mujeeb Ur ^{[1
,2
,3
]}

Yang, Kema ^{[1
]}

Dong, Mengyi ^{[1
]}

Yang, Jing ^{[1
]}

Wang, Mingshu ^{[1
,2
,3
]}

Jia, Renyong ^{[1
,2
,3
]}

Chen, Shun ^{[1
,2
,3
]}

Liu, Mafeng ^{[1
,2
,3
]}

Zhu, Dekang ^{[1
,3
]}

Zhao, Xinxin ^{[1
,2
,3
]}

Yang, Qiao ^{[1
,2
,3
]}

Wu, Ying ^{[1
,2
,3
]}

Zhang, Ling ^{[1
,2
,3
]}

Liu, Yunya ^{[1
,2
,3
]}

Yu, Yanling ^{[1
,2
,3
]}

Tian, Bin ^{[1
,2
,3
]}

Pan, Leichang ^{[1
,2
,3
]}

Chen, Xiaoyue ^{[1
,3
]}

Cheng, Anchun ^{[1
,2
,3
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Avian Dis Res Ctr, Chengdu 611130, Sichuan, Peoples R China

[2] Sichuan Agr Univ, Inst Prevent Vet Med, Chengdu 611130, Sichuan, Peoples R China

[3] Sichuan Agr Univ, Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2019年 / 183卷

关键词：

Escherichia coli; Gene cassettes; Integrons; incF plasmid; Waterfowl birds; ANTIBIOTIC-RESISTANCE GENES; CASSETTE ARRAYS; STRAINS; SPREAD; SWINE; PREVALENCE; PLASMIDS; SPP;

D O I：

10.1016/j.ecoenv.2019.109514

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study was conducted to determine the prevalence of integrons and associated gene cassettes in Escherichia coli (E. coli) isolates from waterfowls in Hainan, China. The antimicrobial resistance profile of the isolates was examined by using disc diffusion test. In addition, PCR, RFLP, plasmid replicon typing and DNA sequencing analyses were used for the characterization of integrase genes (class 1, 2 and 3) and associated gene cassettes. Approximatively, 90% of the isolates were positive for the integrase genes by PCR. Specifically, class 1 and class 2 integrons were found in 252 (81%) and 7 (2.3%) strains, respectively. While 21 (6.7%) isolates were positive for both class 1 and class 2 integrons. However, none of the isolate was positive for the class 3 integrons. In addition, 5 various cassette arrays, dfrA1-orfC, aadA2, aadA1, dfrA1-aadA1, and dfrA1-orfC- aadA1, were found within the variable regions (VRs) of class 1 integron isolates. While only single cassette array, dfrA1-sat2-aadA1, was identified within VRs of class 2 integron isolates. We identified incF plasmid as the most common plasmid type, which was detected in 81 of 243 VRs containing isolates. This study is the first report showing the baseline characteristics of integrons in E. coil isolates from waterfowls in Hainan, China. Our results provide evidence of the waterfowl birds as a reservoir of class 1 and class 2 integrons carrying antibiotic resistance gene cassettes. Therefore, strict preventive measures should be taken to avoid the spread of mobile genetic resistance elements in waterfowls in China.

引用

页数：6

共 36 条

[1]

Alvarez V. E, 2016, BIOL BIOTECHNOLOGY P, P111

[2] Antimicrobial agent exposure and the emergence and spread of resistant microorganisms: issues associated with study design [J].