Zhengella mangrovi gen. nov., sp nov., a novel member of family Phyllobacteriaceae isolated from mangrove sediment

A Gram-negative strain designed X9-2-2(T) was isolated from mangrove sediment in Yunxiao Mangrove National Nature Reserve China. Strain X9-2-2(T) showed less than 96.2% 16S rRNA gene sequence similarity to type strains of species with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences and rpoB protein sequences revealed that strain X9-2-2(T) formed a distinct monophyletic clade within the family Phyllobacteriaceae and clustered distantly with the genera Aliihoeflea Phyllobacterium and Hoeflea. Cells of X9-2-2(T) were rod-shaped motile with subpolar or lateral flagella and facultative anaerobic. Optimal growth occurred at 30-37 degrees C at pH 7 and in the presence of 2% NaCl. The DNA G+C content of strain X9-2-2(T) was 64.9 mol%. The major fatty acids were summed feature 8 (comprising C-18 (: 1)omega 7c and/or C-18 : (1)omega 6c 56.0 %) iso -C-17 (: 0) (9.1 %) and C-12 (: 0) (6.6 %). The predominant respiratory quinone was ubiquinone-10 and the major polar lipids were diphosphatidylglycerol phosphatidylcholine phosphatidylethanolamine phosphatidylglycerol phosphatidylmonomethylethanolamine an unidentified aminolipid and two unidentified polar lipids. According to its morphology physiology fatty acid composition and 16S rRNA gene signature nucleotide patterns strain X9-2-2(T) represents a novel species of a novel genus in the family Phyllobacteriaceae for which the name Zhengella mangrovi gen. nov. sp. nov. is proposed. The type strain is X9-2-2(T) (=MCCC 1K03307(T)=JCM 32107(T)).