Infection of cultured intestinal epithelial cells with severe acute respiratory syndrome coronavirus

被引：63

作者：

Cinatl, J

Hoever, G

Morgenstern, B

Preiser, W

Vogel, JU

Hofmann, WK

Bauer, G

Michaelis, M

Rabenau, HF

Doerr, HW

机构：

[1] Univ Frankfurt Hosp, Inst Med Virol, D-60596 Frankfurt, Germany

[2] Univ Frankfurt Hosp, Dept Haematol & Oncol, D-60596 Frankfurt, Germany

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2004年 / 61卷 / 16期

关键词：

SARS; SARS-CoV; coronavirus; intestine; Caco-2; CL-14; microarray;

D O I：

10.1007/s00018-004-4222-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify a model for the study of intestinal pathogenesis of severe acute respiratory syndrome (SARS) we tested the sensitivity of six human intestinal epithelial cell lines to infection with SARS coronavirus (SARS-CoV). In permissive cell lines, effects of SARS-CoV on cellular gene expression were analysed using high-density oligonucleotide arrays. Caco-2 and CL-14 cell lines were found to be highly permissive to SARS-CoV, due to the presence of angiotensin-converting enzyme 2 as a functional receptor. In both cell lines, SARS-CoV infection deregulated expression of cellular genes which may be important for the intestinal pathogenesis of SARS.

引用

页码：2100 / 2112

页数：13

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