Angiotensin. Activates MCP-1 and Induces Cardiac Hypertrophy and Dysfunction via Toll-like Receptor 4

Aim: Angiotensin. (AngII) produces reactive oxygen species (ROS), thus contributing to the development of cardiac hypertrophy and subsequent heart failure, and stimulates the expression of monocyte chemoattractant protein-1 (MCP-1). In addition, Toll-like receptor 4 (TLR4) is involved in the upregulation of MCP-1. In order to clarify whether TLR4 is involved in the onset of cardiac dysfunction caused by Ang II stimulation, we investigated the effects of TLR4 on oxidative stress, the MCP-1 expression and cardiac dysfunction in mice with AngII-induced hypertension. Methods: TLR4-deficient (Tlr4(lps-d)) and wild-type (WT) mice were randomized into groups treated with Ang., norepinephrine (NE) or a subdepressor dose of the Ang. receptor blocker irbesartan (IRB) and Ang. for two weeks. Results: Ang. and NE similarly increased systolic blood pressure in all drug-treated groups compared to that observed in the control group among both WT and Tlr4(lps-d) mice (p < 0.05). In the WT mice, Ang. induced cardiac hypertrophy as well as vascular remodeling and perivascular fibrosis of the intramyocardial arteries and monocyte/macrophage infiltration in the heart (p < 0.05). Furthermore, Ang. treatment decreased the left ventricular diastolic function and resulted in a greater left ventricular end-systolic dimension (p < 0.05) in addition to producing a five-fold increase in the NADPH oxidase activity, ROS content and MCP-1 expression (p < 0.05). In contrast, the Tlr4lps-d mice showed little effects of Ang. on these indices. In the WT mice, IRB treatment reversed these changes compared to that seen in the mice treated with Ang. alone. NE produced little effect on any of the indices in either the WT or Tlr4lps-d mice. Conclusions: TLR4 may be involved in the processes underlying the increased oxidative stress, selectively activated MCP-1 expression and cardiac hypertrophy and dysfunction seen in cases of Ang.induced hypertension.