Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate

被引：9

作者：

Wang, Xiaoli ^{[1
]}

Wang, Jiao ^{[2
]}

Zhang, Wenmei ^{[1
]}

Li, Boye ^{[1
]}

Zhu, Ying ^{[1
]}

Hu, Qin ^{[1
]}

Yang, Yishu ^{[1
]}

Zhang, Xiaoguang ^{[2
]}

Yan, Hong ^{[1
]}

Zeng, Yi ^{[2
]}

机构：

[1] Beijing Univ Technol, Coll Life Sci & Bioengn, Beijing 100124, Peoples R China

[2] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China

来源：

VIRUSES-BASEL | 2018年 / 10卷 / 05期

基金：

中国国家自然科学基金;

关键词：

human immunodeficiency virus type 1; Keggin polyoxometalate; entry inhibition; CD4; gp41; NHR; IN-VITRO; HIV-1; ENTRY; ANTIVIRAL ACTIVITY; REVERSE-TRANSCRIPTASE; ANTI-HIV-1; AGENTS; MULTIPLE STEPS; VIVO; REPLICATION; HPA-23; ASSAY;

D O I：

10.3390/v10050265

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here, we report the anti-human immunodeficiency virus (HIV) potency and underlying mechanisms of a Keggin polyoxometalate (PT-1, K6HPTi2W10O40). Our findings showed that PT-1 exhibited highly potent effects against a diverse group of HIV type 1 (HIV-1) strains and displayed low cytotoxicity and genotoxicity. The time-addition assay revealed that PT-1 acted at an early stage of infection, and these findings were supported by the observation that PT-1 had more potency against Env-pseudotyped virus than vesicular stomatitis virus glycoprotein (VSVG) pseudotyped virus. Surface plasmon resonance binding assays and flow cytometry analysis showed that PT-1 blocked the gp120 binding site in the CD4 receptor. Moreover, PT-1 bound directly to gp41 NHR (N36 peptide), thereby interrupting the core bundle formation of gp41. In conclusion, our data suggested that PT-1 may be developed as a new anti-HIV-1 agent through its effects on entry inhibition.

引用

页数：15

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