Evaluation of an RSIVD-resistant Trait of Red Sea Bream Pagrus major Broodstock Using DNA-based Pedigree Tracings: A Field Study

被引：9

作者：

Sawayama, Eitaro ^{[1
]}

Takagi, Motohiro ^{[2
]}

机构：

[1] Marua Suisan Co Ltd, R&D Div, Kamijima, Ehime 7942410, Japan

[2] Ehime Univ, Tarumi Branch, South Ehime Fisheries Res Ctr, Matsuyama, Ehime 7908566, Japan

来源：

FISH PATHOLOGY | 2017年 / 52卷 / 01期

关键词：

Pagrus major; red sea bream; RSIVD; pedigree tracing; resistant; family structure; micro-satellite; field study; INFECTIOUS PANCREATIC NECROSIS; ATLANTIC SALMON; LYMPHOCYSTIS DISEASE; IRIDOVIRAL DISEASE; SUSCEPTIBILITY; SELECTION; MARKERS;

D O I：

10.3147/jsfp.52.23

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

In this study, we evaluated the red sea bream iridoviral disease-resistant (RSIVD-resistant) trait of broodstock of red sea bream used in a commercial production based on DNA parentage analysis. We compared family structures of two groups from the same production lot and cultured at two different farms: a population without any outbreak of diseases (farm A) and a high mortality population after an RSIVD outbreak (farm B). The survival percentage of the fish at the farm A and farm B were 86.4% and 20.5%, respectively. Pedigree tracing was conducted on 200 individuals from each farm using eight microsatellite DNA markers among 22 potential breeders (20 dams and 2 sires), and parental pairs at farm A and B were successfully assigned with 93.0 and 90.5%, respectively. After allocating parentage information to each specimen collected from both farms, the estimated survival percentages of each family were calculated. The estimated survival of offspring from a male was 82.3%, and this survival was higher than that from the other male (estimated survival was 2.5%). Also, offspring from a female showed a high survival. These results suggest that some broodstock have resistant traits against RSIVD and shows the potential for developing an RSIVD-resistant strain of red sea bream.

引用

页码：23 / 30

页数：8

共 27 条

[1] Development of a real-time PCR assay for the detection and quantification of red Seabream iridovirus (RSIV) [J].

Caipang, CM ;

Hirono, L ;

Aoki, T .

FISH PATHOLOGY, 2003, 38 (01) :1-7

[2] Parental genetic diversity of brown trout (Salmo trutta m. fario) brood stock affects offspring susceptibility to whirling disease [J].

Eszterbauer, Edit ;

Forro, Barbara ;

Tolnai, Zoltan ;

Guti, Csaba Ferenc ;

Zsigmond, Gergely ;

Hoitsy, Gyoergy ;

Kallert, Dennis Marc .

PARASITES & VECTORS, 2015, 8

[3] Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus) [J].

Fuji, Kanako ;

Hasegawa, Osamu ;

Honda, Kazumitsu ;

Kumasaka, Kiyohiro ;

Sakamoto, Takashi ;

Okamoto, Nobuaki .

AQUACULTURE, 2007, 272 (1-4) :291-295

[4] Identification of a single major genetic locus controlling the resistance to lymphocystis disease in Japanese flounder (Paralichthys olivaceus) [J].

Fuji, Kanako ;

Kobayashi, Kazunobu ;

Hasegawa, Osamu ;

Moura Coimbra, Maria Raquel ;

Sakamoto, Takashi ;

Okamoto, Nobuaki .

AQUACULTURE, 2006, 254 (1-4) :203-210

[5] Microsatellite multiplex panels for population genetic analysis of red sea bream Pagrus major [J].

Gonzalez, Enrique Blanco ;

Aritaki, Masaki ;

Taniguchi, Nobuhiko .

FISHERIES SCIENCE, 2012, 78 (03) :603-611

[6]

Goudet J, 2001, FSTAT PROGRAM ESTIMA

[7] Evidence of recent signatures of selection during domestication in an Atlantic salmon population [J].

Gutierrez, A. P. ;

Yanez, J. M. ;

Davidson, W. S. .

MARINE GENOMICS, 2016, 26 :41-50

[8] Analysis of the incidence of infectious pancreatic necrosis mortality in pedigreed Atlantic salmon, Salmo salar L., populations [J].

Guy, D. R. ;

Bishop, S. C. ;

Brotherstone, S. ;

Hamilton, A. ;

Roberts, R. J. ;

McAndrew, B. J. ;

Woolliams, J. A. .

JOURNAL OF FISH DISEASES, 2006, 29 (11) :637-647

[9] Isolation and application of microsatellite DNA markers for pedigree tracing of seedlings of red sea bream (Pagrus major) [J].

Hatanaka, Akimasa ;

Yamada, Sho-Ichi ;

Sakamoto, Takashi ;

Mitsuboshi, Toru .

JOURNAL OF THE WORLD AQUACULTURE SOCIETY, 2006, 37 (01) :139-143

[10] The susceptibility of Atlantic salmon fry to freshwater infectious pancreatic necrosis is largely explained by a major QTL [J].

Houston, R. D. ;

Haley, C. S. ;

Hamilton, A. ;

Guy, D. R. ;

Mota-Velasco, J. C. ;

Gheyas, A. A. ;

Tinch, A. E. ;

Taggart, J. B. ;

Bron, J. E. ;

Starkey, W. G. ;

McAndrew, B. J. ;

Verner-Jeffreys, D. W. ;

Paley, R. K. ;

Rimmer, G. S. E. ;

Tew, I. J. ;

Bishop, S. C. .

HEREDITY, 2010, 105 (03) :318-327

← 1 2 3 →