Evaluation of enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infections

被引：9
作者：
Huang, Xiaohong
Xuan, Xuenan
Yokoyama, Naoaki
Katayama, Yoshinari
Anzai, Toru
Igarashi, Ikuo
机构：
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Epizoot Res Inst Japan Racing Assoc, Div Microbiol, Kokubunji, Tokyo 3290412, Japan
来源：
VETERINARY PARASITOLOGY | 2006年 / 140卷 / 1-2期
基金：
日本学术振兴会;
关键词：
B; caballi; equi; Bc48; EMA-2t; ELISA;
D O I：
10.1016/j.vetpar.2006.03.013
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Two enzyme-linked immunosorbent assays (ELISA) with recombinant protein as antigens were evaluated by comparison with the indirect fluorescent antibody tests (IFAT) for the detection of specific antibodies to Babesia caballi and Babesia equi, respectively in 380 sera from experimentally infected, uninfected, and field horses. The high concordances of 92.4% (351/380) and 98.2% (373/380) between ELISA and IFAT for B. caballi and B. equi, respectively suggest that ELISA, especially for B. equi infection, could be alternative to the corresponding IFAT for serodiagnoses of equine piroplasmosis, although some improvements are required in ELISA for B. caballi. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：158 / 161
页数：4
相关论文
共 11 条
  • [1] de Waal D. T., 2000, Journal of Protozoology Research, V10, P106
  • [2] HOLBROOK AA, 1969, J AM VET MED ASSOC, V155, P453
  • [3] High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis
    Huang, XH
    Xuan, XN
    Yokoyama, N
    Xu, LS
    Suzuki, H
    Sugimoto, C
    Nagasawa, H
    Fujisaki, K
    Igarashi, I
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (03) : 1147 - 1151
  • [4] Cloning and expression of a 48-kilodalton Babesia caballi merozoite rhoptry protein and potential use of the recombinant antigen in an enzyme-linked immunosorbent assay
    Ikadai, H
    Xuan, XN
    Igarashi, I
    Tanaka, S
    Kanemaru, T
    Nagasawa, H
    Fujisaki, K
    Suzuki, N
    Mikami, T
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) : 3475 - 3480
  • [5] Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rhoptry protein
    Ikadai, H
    Osorio, CR
    Xuan, XN
    Igarashi, I
    Kanemaru, T
    Nagasawa, H
    Fujisaki, K
    Suzuki, N
    Mikami, T
    [J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2000, 30 (05) : 633 - 635
  • [6] Detection of equine antibodies to Babesia caballi by recombinant B-caballi rhoptry-associated protein 1 in a competitive-inhibition enzyme-linked immunosorbent assay
    Kappmeyer, LS
    Perryman, LE
    Hines, SA
    Baszler, TV
    Katz, JB
    Hennager, SG
    Knowles, DP
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (07) : 2285 - 2290
  • [7] Genetic and biochemical analysis of erythrocyte-stage surface antigens belonging to a family of highly conserved proteins of Babesia equi and Theileria species
    Knowles, DP
    Kappmeyer, LS
    Perryman, LE
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 90 (01) : 69 - 79
  • [8] MADDEN PA, 1968, AM J VET RES, V29, P117
  • [9] EVALUATION OF INDIRECT FLUORESCENT-ANTIBODY TEST FOR BABESIA MAJOR AND THEILERIA MUTANS IN BRITAIN
    MORZARIA, SP
    BROCKLESBY, DW
    HARRADINE, DL
    [J]. VETERINARY RECORD, 1977, 100 (23) : 484 - 487
  • [10] SERODIAGNOSIS OF EXPERIMENTAL AND NATURAL BABESIA-EQUI AND BABESIA-CABALLI INFECTIONS
    TENTER, AM
    FRIEDHOFF, KT
    [J]. VETERINARY PARASITOLOGY, 1986, 20 (1-3) : 49 - 61
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