Blue Maize: Morphology and Starch Synthase Characterization of Starch Granule

被引:14
作者
Utrilla-Coello, Rubi G. [1 ]
Agama-Acevedo, Edith [1 ]
Paulina Barba de la Rosa, Ana [2 ]
Martinez-Salgado, Jose L. [2 ]
Rodriguez-Ambriz, Sandra L. [1 ]
Bello-Perez, Luis A. [1 ]
机构
[1] IPN, Ctr Desarrollo Prod Bioticos, Yautepec 62731, Morelos, Mexico
[2] Inst Potosino Invest Cient & Technol, San Luis Potosi 78216, Mexico
关键词
Blue maize; Starch; Morphology; Starch synthases; WAXY LOCUS; PROTEINS; AMYLOPECTIN; ENDOSPERM; ENZYMES; RICE;
D O I
10.1007/s11130-008-0106-8
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The use of pigmented maize varieties has increased due to their high anthocyanins content, but very few studies are reported about the starch properties of these grains. The aim of this work was to isolate the starch granules from pigmented blue maize and carry out the morphological, physicochemical, and biochemical characterization studies. The proximate composition of starch granules showed high protein contents, after purification, the blue maize starch presented lower protein amount than starch from white maize (control). Although the purity of starch granules was increased, the damaged starch (determined for the Maltase cross absence) was also increased. Scanning electron microscopy showed the presence of some pores and channels in the blue maize starch. The electrophoretic protein profiles showed differences in the bands that correspond to the enzymes involved in the starch biosynthesis; these differences could explain the variation in morphological characteristics of blue maize starches against starch from white maize.
引用
收藏
页码:18 / 24
页数:7
相关论文
共 33 条
[1]  
[Anonymous], 2000, APPR METH AN
[2]  
Baldwin PM, 2001, STARCH-STARKE, V53, P475, DOI 10.1002/1521-379X(200110)53:10<475::AID-STAR475>3.0.CO
[3]  
2-E
[4]  
BELLOPEREZ LA, 2005, PLANT FOODS HUM NUTR, V60
[5]   RFLP-BASED GENETIC MAPS OF WHEAT HOMOLOGOUS GROUP-7 CHROMOSOMES [J].
CHAO, S ;
SHARP, PJ ;
WORLAND, AJ ;
WARHAM, EJ ;
KOEBNER, RMD ;
GALE, MD .
THEORETICAL AND APPLIED GENETICS, 1989, 78 (04) :495-504
[6]  
FANNON JE, 1992, CEREAL CHEM, V69, P284
[7]   Anthocyanins from maize (Zea mays) and reed canarygrass (Phalaris arundinacea) [J].
Fossen, T ;
Slimestad, R ;
Andersen, OM .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (05) :2318-2321
[8]   A starch hydrolysis procedure to estimate glycemic index [J].
Goni, I ;
GarciaAlonso, A ;
SauraCalixto, F .
NUTRITION RESEARCH, 1997, 17 (03) :427-437
[9]   Starch biosynthesis: Understanding the functions and interactions of multiple isozymes of starch synthase and branching enzyme [J].
Guan, HP ;
Keeling, PL .
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, 1998, 10 (54) :307-319
[10]   Chemical composition and in vitro starch digestibility of pigmented corn tortilla (vol 87, pg 2482, 2007) [J].
Hernandez-Uribe, Juan Pablo ;
Agama-Acievedo, Edith ;
Islas-Hernandez, Jose Juan ;
Tovar, Juscelino ;
Bello-Perez, Luis A. .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2007, 87 (14) :2733-2733