Real-time PCR for quantification of eleven individual Fusarium species in cereals

被引：227

作者：

Nicolaisen, Mogens ^{[1
]}

Supronien, Skaidre

Nielsen, Linda Kaergaard ^{[1
]}

Lazzaro, Irene ^{[1
]}

Spliid, Niels Henrik ^{[1
]}

Justesen, Annemarie Feier ^{[1
]}

机构：

[1] Aarhus Univ, Fac Agr Sci, Dept Integrated Pest Management, DK-4200 Slagelse, Denmark

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 76卷 / 03期

关键词：

Fusarium; Mycotoxin; Real-time PCR; Maize; Wheat; HEAD BLIGHT; MYCOTOXINS; WHEAT; DNA;

D O I：

10.1016/j.mimet.2008.10.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Contamination of cereals with Fusarium species is one of the major sources of mycotoxins in food and feed. Quantification of biomass of Fusarium species is essential to understand the interactions of individual species in disease development. In this study quantitative real-time PCR assays based on the elongation factor 1 alpha (EF1 alpha) gene for the 11 Fusarium species F. graminearum, F. culmorum, F. poae, F. langsethiae, F. sporotrichioides, F. equiseti, F. tnicinctum, F. avenaceum, F. verticillioides, F. subglutinans and F. proliferatum were developed and tested on 24 wheat and 24 maize field samples. The assays were found to be specific and sensitive. Generally, the results from the quantitative real-time PCR assays corresponded well with mycotoxin data of the field samples. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：234 / 240

页数：7

共 50 条

[1] Real-time PCR for quantification of toxigenic Fusarium species in barley and malt
Sarlin, Tuija
Yli-Mattila, Tapani
Jestoi, Marika
Rizzo, Aldo
Paavanen-Huhtala, Sari
Haikara, Auli
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2006, 114 (04) : 371 - 380
[2] Real-time PCR for Quantification of Toxigenic Fusarium Species in Barley and Malt
Tuija Sarlin
Tapani Yli-Mattila
Marika Jestoi
Aldo Rizzo
Sari Paavanen-Huhtala
Auli Haikara
European Journal of Plant Pathology, 2006, 114 : 371 - 380
[3] Real-time PCR quantification of Fusarium avenaceum in soil and seeds
Pollard, Anne T.
Okubara, Patricia A.
JOURNAL OF MICROBIOLOGICAL METHODS, 2019, 157 : 21 - 30
[4] Detection and Quantification of Individual Fungal Species in Fusarium Disease Complexes of Cereals by Polymerase Chain Reaction (PCR)
P. Nicholson
F. Doohan
H. N. Rezanoor
D. Simpson
P. H. Smith
A. Turner
G. Weston
Cereal Research Communications, 1997, 25 (3) : 477 - 482
[5] Detection and quantification of individual fungal species in Fusarium disease complexes of cereals by polymerase chain reaction (PCR)
Nicholson, P
Doohan, F
Rezanoor, HN
Simpson, D
Smith, PH
Turner, A
Weston, G
CEREAL RESEARCH COMMUNICATIONS, 1997, 25 (03) : 477 - 482
[6] Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe
Reischer, GH
Lemmens, M
Farnleitner, A
Adler, A
Mach, RL
JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (01) : 141 - 146
[7] Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize
Atoui, Ali
El Khoury, Andre
Kallassy, Mireille
Lebrihi, Ahmed
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2012, 154 (1-2) : 59 - 65
[8] Multiplex real-time PCR for detection and quantification of mycotoxigenic Aspergillus, Penicillium and Fusarium
Suanthie, Yenny
Cousin, Maribeth A.
Woloshuk, Charles P.
JOURNAL OF STORED PRODUCTS RESEARCH, 2009, 45 (02) : 139 - 145
[9] Species-specific real-time PCR for quantification of Toxoplasma gondii
Vorwerk, N
von Samson-Himmelstjerna, G
Tenter, AM
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2004, 293 : 45 - 45
[10] Reliable quantification of rhinovirus species C using real-time PCR
Sikazwe, Chisha T.
Chidlow, Glenys R.
Imrie, Allison
Smith, David W.
JOURNAL OF VIROLOGICAL METHODS, 2016, 235 : 65 - 72

← 1 2 3 4 5 →