Use of DNA transposons for functional genetic screens in mouse models of cancer

被引：5

作者：

Bermejo-Rodriguez, Camino ^{[1
]}

Perez-Mancera, Pedro A. ^{[1
,2
]}

机构：

[1] Univ Cambridge, Canc Res UK Cambridge Inst, Li Ka Shing Ctr, Cambridge CB2 0RE, England

[2] Univ Liverpool, Dept Mol & Clin Canc Med, Natl Inst Hlth Res, Liverpool Pancreas Biomed Res Unit, Liverpool L69 3GA, Merseyside, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 35卷

关键词：

SLEEPING-BEAUTY; INSERTIONAL MUTAGENESIS; HEPATOCELLULAR-CARCINOMA; INTESTINAL TUMORIGENESIS; IDENTIFIES GENES; LIVER-CANCER; MICE; DISCOVERY; PIGGYBAC; SYSTEM;

D O I：

10.1016/j.copbio.2015.05.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cancer is a very heterogeneous disease with complex genetic interactions. In recent years, the systematic sequencing of cancer genomes has provided information to design personalized therapeutic interventions. However, the complexity of cancer genomes commonly makes it difficult to identify specific genes involved in tumour development or therapeutic responsiveness. The generation of mouse models of cancer using transposon-mediated approaches has provided a powerful tool to unveil the role of key genes during cancer development. Here we will discuss how the use of forward and reverse genetic approaches mediated by DNA transposons can support the investigation of cancer pathogenesis, including the identification of cancer promoting mechanisms and potential therapeutic targets.

引用

页码：103 / 110

页数：8

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