Specific inhibitory effect of hybrid liposomes on the growth of hybridoma cells in vitro

Remarkably high inhibitory effects of the hybrid liposomes composed of L-alpha-dimyristoyl-phosphatidylcholine (DMPC) and polyoxyethylenealkyl ether (C-14(EO)(n), n = 6-8 and C-12(EO)(n), n = 8-12)) on the growth of human lymphoma-human B-lymphocyte hybridoma (HF) cells in vitro were obtained. The hybrid liposomes composed of 90 mol% DMPC/10 mol% C-14(EO)(n) (n = 6-8) or C-12(EO)(n) (n = 8-12) were more fluid as compared with 90mol% DMPC/10 mol% C-14(EO)(4) or C-12(EO)(n) (n = 4, 23) hybrid liposomes on the basis of fluorescence polarization measurements. These results suggest that the inhibitory effects of the hybrid liposomes on the growth of HF cells should be related to the membrane fluidity. No toxicity to normal rats in vivo was observed in the experiment using 90mol% DMPC/10mol% C-14(EO)(7) Or 90mol% DMPC/10 mol% C-12(EO)(12) hybrid liposomes.