How Are Cytochrome b561 Electron Currents Controlled by Membrane Voltage and Substrate Availability?

被引：17

作者：

Picco, Cristiana ^{[1
]}

Scholz-Starke, Joachim ^{[1
]}

Naso, Alessia ^{[1
]}

Preger, Valeria ^{[2
]}

Sparla, Francesca ^{[2
]}

Trost, Paolo ^{[2
]}

Carpaneto, Armando ^{[1
]}

机构：

[1] CNR, Inst Biophys, I-16149 Genoa, Italy

[2] Univ Bologna, Dept Pharm & Biotechnol FaBiT, I-40126 Bologna, Italy

来源：

ANTIOXIDANTS & REDOX SIGNALING | 2014年 / 21卷 / 03期

关键词：

NADPH OXIDASE; DEPENDENCE; REDUCTASE;

D O I：

10.1089/ars.2013.5809

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct recordings of electron currents mediated by cytochromes b561 (CYB561) are not available yet, despite the importance of these proteins in a variety of physiological functions, including neurotransmitter synthesis and dietary iron uptake. Here, we used the two-electrode voltage-clamp technique applied to Xenopus oocytes to demonstrate, for the first time, the generation of electron currents by a Drosophila member of the CYB561 superfamily named stromal cell-derived receptor 2 (SDR2). This experimental method, along with the theoretical development of a three-state kinetic model, supports the hypothesis that electron donor/acceptor concentrations and transmembrane voltage mutually control SDR2-mediated electron transport activity in a complex but predictable manner.

引用

收藏

页码：384 / 391

页数：8

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