Analysis of N-acylhomoserine lactone dynamics in continuous cultures of Pseudomonas putida IsoF by use of ELISA and UHPLC/qTOF-MS-derived measurements and mathematical models

被引：16

作者：

Buddrus-Schiemann, Katharina ^{[1
]}

Rieger, Martin ^{[1
]}

Muehlbauer, Marlene ^{[1
]}

Barbarossa, Maria Vittoria ^{[2
]}

Kuttler, Christina ^{[3
]}

Hense, Burkhard A. ^{[4
]}

Rothballer, Michael ^{[1
]}

Uhl, Jenny ^{[5
]}

Fonseca, Juliano R. ^{[5
]}

Schmitt-Kopplin, Philippe ^{[5
,6
]}

Schmid, Michael ^{[1
]}

Hartmann, Anton ^{[1
]}

机构：

[1] German Res Ctr Environm Hlth GmbH, Res Unit Microbe Plant Interact, Helmholtz Zentrum Munchen, D-85764 Neuherberg, Germany

[2] Univ Szeged, Bolyai Inst, H-6720 Szeged, Hungary

[3] Tech Univ Munich, Ctr Math Sci, D-85748 Garching, Germany

[4] German Res Ctr Environm Hlth GmbH, Inst Computat Biol, Helmholtz Zentrum Munchen, D-85764 Neuherberg, Germany

[5] German Res Ctr Environm Hlth GmbH, Res Unit Analyt BioGeoChem, Helmholtz Zentrum Munchen, D-85764 Neuherberg, Germany

[6] Tech Univ Munich, Alte Akad 10, D-85354 Freising Weihenstephan, Germany

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2014年 / 406卷 / 25期

基金：

欧洲研究理事会;

关键词：

Pseudomonas putida IsoF; Continuous culture; N-acylhomoserine lactones; Mathematical modelling; ELISA; Quorum sensing; PERFORMANCE LIQUID-CHROMATOGRAPHY; ACYL HOMOSERINE LACTONES; QUORUM-SENSING SYSTEM; BACILLUS-SUBTILIS; BIOFILM FORMATION; PUBLIC-GOODS; AERUGINOSA; COMMUNICATION; BACTERIA; IDENTIFICATION;

D O I：

10.1007/s00216-014-8063-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this interdisciplinary approach, the dynamics of production and degradation of the quorum sensing signal 3-oxo-decanoylhomoserine lactone were studied for continuous cultures of Pseudomonas putida IsoF. The signal concentrations were quantified over time by use of monoclonal antibodies and ELISA. The results were verified by use of ultra-high-performance liquid chromatography. By use of a mathematical model we derived quantitative values for non-induced and induced signal production rate per cell. It is worthy of note that we found rather constant values for different rates of dilution in the chemostat, and the values seemed close to those reported for batch cultures. Thus, the quorum-sensing system in P. putida IsoF is remarkably stable under different environmental conditions. In all chemostat experiments, the signal concentration decreased strongly after a peak, because emerging lactonase activity led to a lower concentration under steady-state conditions. This lactonase activity probably is quorum sensing-regulated. The potential ecological implication of such unique regulation is discussed.

引用

页码：6373 / 6383

页数：11

共 44 条

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