Anti-cancer Effects of Fucoxanthin on Human Glioblastoma Cell Line

被引:25
作者
Lopes, Flavia Garcia [1 ]
Oliveira, Karen Andrineia [2 ]
Lopes, Rafael Garcia [3 ]
Poluceno, Gabriela Godoy [2 ]
Simioni, Carmen [4 ]
Pescador, Gabriel da Silva [5 ]
Bauer, Claudia Marlene [6 ]
Maraschin, Marcelo [6 ]
Derner, Roberto Bianchini [3 ]
Garcez, Ricardo Castilho [5 ]
Tasca, Carla Ines [2 ]
Nedel, Claudia Beatriz [1 ,2 ]
机构
[1] Univ Fed Santa Catarina, Lab Biol Celular Gliomas, Programa Posgrad Biol Celular & Desenvolvimento, Florianopolis, SC, Brazil
[2] Univ Fed Santa Catarina, Lab Neuroquim 4, Programa Posgrad Bioquim, Florianopolis, SC, Brazil
[3] Univ Fed Santa Catarina, Lab Cult Algas, Florianopolis, SC, Brazil
[4] Univ Fed Santa Catarina, Lab Biol Celular Vegetal, Programa Posgrad Biol Celular & Desenvolvimento, Florianopolis, SC, Brazil
[5] Univ Fed Santa Catarina, Lab Celulas Tronco & Regeneracao Tecidual, Programa Posgrad Biol Celular & Desenvolvimento, Florianopolis, SC, Brazil
[6] Univ Fed Santa Catarina, Lab Morfogenese & Bioquim Vegetal, Florianopolis, SC, Brazil
关键词
Glioblastoma; fucoxanthin; apoptosis; migration; invasion; angiogenesis; CYCLE ARREST; CAROTENOID FUCOXANTHIN; EXTRACELLULAR-MATRIX; UP-REGULATION; CANCER; APOPTOSIS; PROLIFERATION; ANGIOGENESIS; EPIDEMIOLOGY; MIGRATION;
D O I
10.21873/anticanres.14703
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background/Aim: Glioblastomas (GBMs) are the most malignant primary brain tumor. New treatment strategies against the disease are urgently needed, as therapies are not completely efficient. In this study, we evaluated the antitumorigenic activity of the carotenoid fucoxanthin (Fx) on human GBM cells in vitro. Materials and Methods: GBM1 cell viability and proliferation was assessed by MTT reduction, Ki67 and single cell cloning assays. GBM1 migration and invasion were analyzed by wound healing and Transwell assays. Apoptosis and necrosis were analyzed by flow cytometry, and the mitochondrial membrane potential (Delta Psi m) by the selective fluorescent dye tetramethylrhodamine ethyl ester. Cell morphology was analyzed through scanning electron microscopy and transmission electron microscopy. Fx anti-angiogenic effect was assessed by the CAM ex ovo assay. Results: Fx decreased cell viability in a concentration-dependent manner 40-100 mu M) in GBM1, A172 and C6 cell lines and was not cytotoxic to murine astrocytes. In addition, Fx inhibited the proliferation and clonogenic potential, and decreased migration and invasion of GBM1 cells. Furthermore, Fx induced apoptosis, loss of Delta Psi m and ultrastructural alterations in GBM1. Fx-treated GBM1 cells-conditioned medium reduced the quail yolk membrane vascularity. Conclusion: Fx induces cytotoxicity, anti-proliferative, anti-invasive and anti-angiogenic effects on GBM1 cells.
引用
收藏
页码:6799 / 6815
页数:17
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