Discovery of novel inhibitors of vascular endothelial growth factor-A-Neuropilin-1 interaction by structure-based virtual screening

被引:37
作者
Starzec, Anna [1 ]
Miteva, Maria A. [2 ,3 ]
Ladam, Patrick [4 ]
Villoutreix, Bruno O. [2 ,3 ]
Perret, Gerard Y. [1 ]
机构
[1] Univ Paris 13, Sorbonne Paris Cite, EA4222, F-93017 Bobigny, France
[2] Univ Paris Diderot, Sorbonne Paris Cite, Inserm UMR S 973, F-75013 Paris, France
[3] INSERM, U973, F-75205 Paris, France
[4] Univ Paris 13, Sorbonne Paris Cite, CSPBAT, F-93017 Bobigny, France
关键词
Neuropilin; VEGF; Angiogenesis; Protein-protein interactions inhibitors; Structure-based virtual screening; IN-SILICO; 3D CONFORMATION; ANTI-VEGF; LIGAND; NEUROPILIN-1; BINDING; GENERATOR; DOCK;
D O I
10.1016/j.bmc.2014.05.068
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Neuropilin-1 (NRP-1), one of the most important co-receptors of vascular endothelial growth factor-A (VEGF-A), increases its angiogenic action in several chronic diseases including cancer by increasing the activity of associated tyrosine kinase receptors, VEGFR1 and VEGFR2. Binding of VEGF-A to NRP-1 plays a critical role in pathological angiogenesis and tumor progression. Today, targeting this interaction is a validated approach to fight against angiogenesis-dependent diseases. Only anti-NRP-1 antibodies, peptide and peptidomimetic drug-candidates or hits have been developed thus far. In order to identify potent orally active small organic molecules various experimental and in silico approaches can be used. Here we report, novel promising small drug-like molecules disrupting the binding of VEGF-A(165) to NRP-1. We carried out structure-based virtual screening experiments using the ChemBridge compound collection on the VEGF-A(165) binding pocket of NRP-1. After docking and two rounds of similarity search computations, we identified 4 compounds that inhibit the biotinylated VEGF-A(165) binding to recombinant NRP-1 with K-i of about 10 mu M. These compounds contain a common chlorobenzyloxy alkyloxy halogenobenzyl amine scaffold that can serve as a base for further development of new NRP-1 inhibitors. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4042 / 4048
页数:7
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