A novel Pseudomonas aeruginosa Bacteriophage, Ab31, a Chimera Formed from Temperate Phage PAJU2 and P. putida Lytic Phage AF: Characteristics and Mechanism of Bacterial Resistance

被引：24

作者：

Latino, Libera ^{[1
,2
]}

Essoh, Christiane ^{[1
,2
]}

Blouin, Yann ^{[1
,2
]}

Hoang Vu Thien ^{[3
]}

Pourcel, Christine ^{[1
,2
]}

机构：

[1] Univ Paris 11, Inst Genet & Microbiol, UMR 8621, F-91405 Orsay, France

[2] CNRS, F-91405 Orsay, France

[3] Hop Armand Trousseau, AP HP, Paris, France

来源：

PLOS ONE | 2014年 / 9卷 / 04期

关键词：

CYSTIC-FIBROSIS; MUCOID CONVERSION; GENOMIC ANALYSIS; GENE-EXPRESSION; LAMBDA-RECEPTOR; VIRULENCE; SEQUENCE; PROTEIN; PERSISTENCE; EVOLUTION;

D O I：

10.1371/journal.pone.0093777

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel temperate bacteriophage of Pseudomonas aeruginosa, phage vB_PaeP_Tr60_Ab31 (alias Ab31) is described. Its genome is composed of structural genes related to those of lytic P. putida phage AF, and regulatory genes similar to those of temperate phage PAJU2. The virion structure resembles that of phage AF and other lytic Podoviridae (S. enterica Epsilon 15 and E. coli phiv10) with similar tail spikes. Ab31 was able to infect P. aeruginosa strain PA14 and two genetically related strains called Tr60 and Tr162, out of 35 diverse strains from cystic fibrosis patients. Analysis of resistant host variants revealed different phenotypes, including induction of pigment and alginate overproduction. Whole genome sequencing of resistant variants highlighted the existence of a large deletion of 234 kbp in two strains, encompassing a cluster of genes required for the production of CupA fimbriae. Stable lysogens formed by Ab31 in strain Tr60, permitted the identification of the insertion site. During colonization of the lung in cystic fibrosis patients, P. aeruginosa adapts by modifying its genome. We suggest that bacteriophages such as Ab31 may play an important role in this adaptation by selecting for bacterial characteristics that favor persistence of bacteria in the lung.

引用

页数：16

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