Electrochemical aptamer scaffold biosensors for detection of botulism and ricin toxins

被引：30

作者：

Fetter, Lisa ^{[1
]}

Richards, Jonathan ^{[1
]}

Daniel, Jessica ^{[1
]}

Roon, Laura ^{[1
]}

Rowland, Teisha J. ^{[2
,3
]}

Bonham, Andrew J. ^{[1
]}

机构：

[1] Metropolitan State Univ Denver, Dept Chem, Denver, CO 80204 USA

[2] Univ Colorado, Cardiovasc Inst, Aurora, CO 80045 USA

[3] Univ Colorado, Adult Med Genet Program, Aurora, CO 80045 USA

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 82期

关键词：

GENERATION; SELECTION; BINDING; BLOOD;

D O I：

10.1039/c5cc05933j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein toxins present considerable health risks, but detection often requires laborious analysis. Here, we developed electrochemical aptamer biosensors for ricin and botulinum neurotoxins, which display robust and specific signal at nanomolar concentrations and function in dilute serum. These biosensors may aid future efforts for the rapid diagnosis of toxins.

引用

页码：15137 / 15140

页数：4

共 28 条

[21] Continuous, Real-Time Monitoring of Cocaine in Undiluted Blood Serum via a Microfluidic, Electrochemical Aptamer-Based Sensor [J].

Swensen, James S. ;

Xiao, Yi ;

Ferguson, Brian S. ;

Lubin, Arica A. ;

Lai, Rebecca Y. ;

Heeger, Alan J. ;

Plaxco, Kevin W. ;

Soh, H. Tom. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (12) :4262-4266

[22] Single microbead SELEX for efficient ssDNA aptamer generation against botulinum neurotoxin [J].

Tok, Jeffrey B. -H. ;

Fischer, Nicholas O. .

CHEMICAL COMMUNICATIONS, 2008, (16) :1883-1885

[23] A Mechanistic Study of Electron Transfer from the Distal Termini of Electrode-Bound, Single-Stranded DNAs [J].

Uzawa, Takanori ;

Cheng, Ryan R. ;

White, Ryan J. ;

Makarov, Dmitrii E. ;

Plaxco, Kevin W. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (45) :16120-16126

[24] Bioelectrochemical Switches for the Quantitative Detection of Antibodies Directly in Whole Blood [J].

Vallee-Belisle, Alexis ;

Ricci, Francesco ;

Uzawa, Takanori ;

Xia, Fan ;

Plaxco, Kevin W. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (37) :15197-15200

[25] Atoxic Derivative of Botulinum Neurotoxin A as a Prototype Molecular Vehicle for Targeted Delivery to the Neuronal Cytoplasm [J].

Vazquez-Cintron, Edwin J. ;

Vakulenko, Maksim ;

Band, Philip A. ;

Stanker, Larry H. ;

Johnson, Eric A. ;

Ichtchenko, Konstantin .

PLOS ONE, 2014, 9 (01)

[26] High-Resolution Single-Molecule Recognition Imaging of the Molecular Details of Ricin-Aptamer Interaction [J].

Wang, Bin ;

Guo, Cunlan ;

Zhang, Mengmeng ;

Park, Bosoon ;

Xu, Bingqian .

JOURNAL OF PHYSICAL CHEMISTRY B, 2012, 116 (17) :5316-5322

[27] Electrochemical biosensors: Towards point-of-care cancer diagnostics [J].

Wang, J .

BIOSENSORS & BIOELECTRONICS, 2006, 21 (10) :1887-1892

[28] Aptamer-based electrochemical biosensor for Botulinum neurotoxin [J].

Wei, Fang ;

Ho, Chih-Ming .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 393 (08) :1943-1948

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