Preparation of Fucoxanthin-Loaded Nanoparticles Composed of Casein and Chitosan with Improved Fucoxanthin Bioavailability

被引：72
作者：
Koo, Song Yi [1 ]
Mok, Il-Kyoon [1 ,2 ]
Pan, Cheol-Ho [1 ]
Kim, Sang Min [1 ,3 ]
机构：
[1] KIST Gangneung Inst Nat Prod, Syst Biotechnol Res Ctr, Kangnung 25451, Gangwon Do, South Korea
[2] Gangneung Wonju Natl Univ, Dept Food Proc & Distribut, Kangnung 210702, Gangwon Do, South Korea
[3] KIST Gangneung Inst Nat Prod, Convergence Res Ctr Smart Farm Solut, Kangnung 25451, Gangwon Do, South Korea
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2016年 / 64卷 / 49期
关键词：
bioavailability; casein; chitosan; fucoxanthin; nanoparticles; Phaeodactylum tricornutum; IN-VIVO PHARMACOKINETICS; SODIUM CASEINATE; DRUG-DELIVERY; STABILITY; VITRO; CURCUMIN; FORMULATION; NANOGELS; SYSTEMS; CARRIER;
D O I：
10.1021/acs.jafc.6b04376
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
To facilitate the utilization of fucoxanthin (FX), a valuable marine carotenoid, in the food industry, FX-loaded casein nanoparticles (FX-CN) and chitosan-coated FX-CN (FX-CS-CN) were developed using the FX-enriched fraction from Phaeodactylum tricornutum. Two nanoscale particles (237 +/- 13 nm for FX-CN and 277 +/- 26 nm for FX-CN-CN) with spherical and smooth surfaces showed over 71% encapsulation efficiency and polydispersity index (PDI) value of 0.31-0.39 in water. Owing to the chitosan coating, FX-CS-CN showed a positive zeta potential (24.00 mV), whereas that of FX-CN was negative (-12.87 mV). In vitro simulated digestion demonstrated better FX bioaccessibility from the nanoparticles versus P. tricornutum powder (Pt-powder) and from FX-CN versus FX-CS-CN. However, in C57BL/6 mice, fucoxanthinol absorption to the blood circulation was two times higher for FX-CS-CN versus FX-CN, possibly due to increased retention or adsorption to mucin by the cationic biopolymer in the chitosan-coated particles. These results demonstrate that FX-CS-CN can enable the application of FX, with improved bioavailability and water dispersibility, in the food industry.
引用
收藏
页码：9428 / 9435
页数：8
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