Comparative analyses among the Trichomonas vaginalis, Trichomonas tenax, and Tritrichomonas foetus 5S ribosomal RNA genes

被引：12

作者：

Lilia Torres-Machorro, Ana ^{[1
]}

Hernandez, Roberto ^{[1
]}

Alderete, John F. ^{[2
]}

Lopez-Villasenor, Imelda ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Inst Invest Biomed, Mexico City 04510, DF, Mexico

[2] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA

来源：

CURRENT GENETICS | 2009年 / 55卷 / 02期

关键词：

Ribosome; Intergenic region; RNA polymerase III; Ubiquitin; Repeated sequences; Chromosome; SEQUENCE-ANALYSIS; GENOME; PLASMODIUM; EVOLUTION; PROTOZOA; REGIONS; LINKAGE; DNA;

D O I：

10.1007/s00294-009-0237-y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The 5S ribosomal RNA (5S rRNA) is an essential component of ribosomes. Throughout evolution, variation is found among 5S rRNA genes regarding their chromosomal localization, copy number, and intergenic regions. In this report, we describe and compare the gene sequences, motifs, genomic copy number, and chromosomal localization of the Trichomonas vaginalis, Trichomonas tenax, and Tritrichomonas foetus 5S rRNA genes. T. vaginalis and T. foetus have a single type of 5S rRNA-coding region, whereas two types were found in T. tenax. The sequence identities among the three organisms are between 94 and 97%. The intergenic regions are more divergent in sequence and size with characteristic species-specific motifs. The T. foetus 5S rRNA gene has larger and more complex intergenic regions, which contain either an ubiquitin gene or repeated sequences. The 5S rRNA genes were located in Trichomonads chromosomes by fluorescent in situ hybridization.

引用

页码：199 / 210

页数：12

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