γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology

被引：64
作者：
Geric, Marko [1 ]
Gajski, Goran [1 ]
Garaj-Vrhovac, Vera [1 ]
机构：
[1] Inst Med Res & Occupat Hlth, Zagreb 10000, Croatia
来源：
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2014年 / 105卷
关键词：
gamma-H2AX foci assay; Genotoxicity; Double-strand breaks; Cytostatic pharmaceuticals; Environmental concentrations; HISTONE H2AX PHOSPHORYLATION; AUTOMATED BIODOSIMETRY TOOL; STALLED REPLICATION FORKS; TANDEM MASS-SPECTROMETRY; DAMAGE RESPONSE; WASTE-WATER; IN-VITRO; INDIVIDUAL RADIOSENSITIVITY; GENOTOXICITY ASSESSMENT; MICRONUCLEUS ASSAY;
D O I：
10.1016/j.ecoenv.2014.03.035
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The visualisation of DNA damage response proteins enables the indirect measurement of DNA damage. Soon after the occurrence of a DNA double-strand break (DSB), the formation of gamma-H2AX histone variants is to be expected. This review is focused on the potential use of the gamma-H2AX foci assay in assessing the genotoxicity of environmental contaminants including cytostatic pharmaceuticals, since standard methods may not be sensitive enough to detect the damaging effect of low environmental concentrations of such drugs. These compounds are constantly released into the environment, potentially representing a threat to water quality, aquatic organisms, and, ultimately, human health. Our review of the literature revealed that this method could be used in the biomonitoring and risk assessment of aquatic systems affected by wastewater from the production, usage, and disposal of cytostatic pharmaceuticals. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：13 / 21
页数：9
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