A metabolomics-based approach for predicting stages of chronic kidney disease

被引：33

作者：

Kobayashi, Toshihiro ^{[1
]}

Yoshida, Tatsunari ^{[2
]}

Fujisawa, Tatsuya ^{[3
]}

Matsumura, Yuriko ^{[1
]}

Ozawa, Toshihiko ^{[1
,4
]}

Yanai, Hiroyuki ^{[1
]}

Iwasawa, Atsuo ^{[1
]}

Kamachi, Toshiaki ^{[1
]}

Fujiwara, Kouichi ^{[3
]}

Kohno, Masahiro ^{[1
]}

Tanaka, Noriaki ^{[3
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biochem, Dept Bioengn, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[2] Shimadzu Co Ltd, Global Applicat Dev Ctr, Nakagyo Ku, Kyoto 6048511, Japan

[3] Kiyokai Tanaka Kitanoda Hosp, Higashi Ku, Sakai, Osaka 5998123, Japan

[4] Yokohama Coll Pharm, Totsuka Ku, Yokohama, Kanagawa 2450066, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 445卷 / 02期

关键词：

CKD; Cystatin C; GFR; LC/MS; OPLS; CHRONIC-RENAL-FAILURE; MASS-SPECTROMETRY; UREMIC TOXINS; CYSTATIN C; PERFORMANCE; PLASMA; RISK;

D O I：

10.1016/j.bbrc.2014.02.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chronic kidney disease (CKD) is a major epidemiologic problem and a risk factor for cardiovascular events and cerebrovascular accidents. Because CKD shows irreversible progression, early diagnosis is desirable. Renal function can be evaluated by measuring creatinine-based estimated glomerular filtration rate (eGFR). This method, however, has low sensitivity during early phases of CKD. Cystatin C (CysC) may be a more sensitive predictor. Using a metabolomic method, we previously identified metabolites in CKD and hemodialysis patients. To develop a new index of renal hypofunction, plasma samples were collected from volunteers with and without CKD and metabolite concentrations were assayed by quantitative liquid chromatography/mass spectrometry. These results were used to construct a multivariate regression equation for an inverse of CysC-based eGFR, with eGFR and CKD stage calculated from concentrations of blood metabolites. This equation was able to predict CKD stages with 81.3% accuracy (range, 73.9-87.0% during 20 repeats). This procedure may become a novel method of identifying patients with early-stage CKD. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：412 / 416

页数：5

共 17 条

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