Effect of callus induction media on morphology of embryogenic calli in rice genotypes

被引：41

作者：

Visarada, KBRS

Sailaja, M

Sarma, NP

机构：

[1] Directorate Rice Res, Hyderabad 500030, Andhra Pradesh, India

[2] Natl Res Ctr Sorghum, Hyderabad 500030, Andhra Pradesh, India

来源：

BIOLOGIA PLANTARUM | 2002年 / 45卷 / 04期

关键词：

medium composition; Oryza sativa; regeneration;

D O I：

10.1023/A:1022323221513

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Effects of four culture media on callus induction, regeneration and number of plants per unit culture were studied with mature seeds from five indica rice genotypes as explants. Based on the morphology, the calli were classified into four types as I to IV. Type I and type 11 are most suited to initiate suspension cultures or as target material for transformation. Number of plants regenerated per unit culture, formation of easily dissociating cell clusters and frequency of type I and type II calli were highest on NBKNB medium. Thus NBKNB medium is suitable for in vitro culture of even the hitherto recalcitrant indica genotypes.

引用

收藏

页码：495 / 502

页数：8

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