Inhibitory effect of sesaminol glucosides on lipopolysaccharide-induced NF-κB activation and target gene expression in cultured rat astrocytes

The inflammatory reaction plays an important role in the pathogenesis of the neurodegenerative disorder including Alzheimer's disease (AD). Sesame lignan compounds such as sesaminol glucosides (SG) exhibit a range of pharmacological activities including anti-oxidative and anti-inflammatory action. In this study, we tried to elucidate possible effects of SG on lipopolysaccharide (LPS)-induced inflammatory reaction and its underlying mechanism in cultured astrocytes. SG (10-100 mu g/ml) inhibited LPS-induced generation of nitric oxide (NO) and reactive oxygen species (ROS), as well as inhibited LPS-induced cytosolic phospholipase A(2) (cPLA(2)), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expression dose-dependently. This inhibitory effect of SG on NO and ROS generation was enforced by addition of glutathione (GSH) in culture. In addition, SG prevented LPS-induced DNA binding and transcriptional activity of nuclear factor KappaB (NF)-kappa B. Consistent with the inhibitory effect on NF-kappa B activity, SG inhibits phosphorylation and degradation of inhibitory KappaB (I kappa B), thereby translocation of p50 of NF-kappa B. These data show that SG has an anti-inflammatory effect through inhibition of NF-kappa B, and may be a useful agent for prevention of inflammatory disease like AD. (c) 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.