Schisandrin B Induces Apoptosis and Cell Cycle Arrest of Gallbladder Cancer Cells

被引:39
作者
Xiang, Shan-Shan [1 ,2 ,3 ]
Wang, Xu-An [1 ,2 ,3 ]
Li, Huai-Feng [1 ,2 ,3 ]
Shu, Yi-Jun [1 ,2 ,3 ]
Bao, Run-Fa [1 ,2 ,3 ]
Zhang, Fei [1 ,2 ,3 ]
Cao, Yang [1 ,2 ,3 ]
Ye, Yuan-Yuan [1 ,2 ,3 ]
Weng, Hao [1 ,2 ,3 ]
Wu, Wen-Guang [1 ,2 ,3 ]
Mu, Jia-Sheng [1 ,2 ,3 ]
Wu, Xiang-Song [1 ,2 ,3 ]
Li, Mao-Lan [1 ,2 ,3 ]
Hu, Yun-Ping [1 ,2 ,3 ]
Jiang, Lin [1 ,2 ,3 ]
Tan, Zhu-Jun [1 ,2 ,3 ]
Lu, Wei [1 ,2 ,3 ]
Liu, Feng [4 ]
Liu, Ying-Bin [1 ,2 ,3 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Dept Gen Surg, Shanghai 200092, Peoples R China
[2] Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Lab Gen Surg, Shanghai 200092, Peoples R China
[3] Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Inst Biliary Tract Dis, Shanghai 200092, Peoples R China
[4] Nanchang Univ, Affiliated Hosp 1, Emergency Unit, Nanchang 330006, Peoples R China
基金
中国国家自然科学基金;
关键词
schisandrin B; gallbladder cancer; apoptosis; cell cycle arrest; mitochondrial pathway; CARCINOMA METASTASIS; KINASE-ACTIVITY; DNA-DAMAGE; IN-VITRO; INHIBITION; PROLIFERATION; INDUCTION; PATHWAYS; INVASION; PROTEIN;
D O I
10.3390/molecules190913235
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gallbladder cancer, with high aggressivity and extremely poor prognosis, is the most common malignancy of the bile duct. The main objective of the paper was to investigate the effects of schisandrin B (Sch B) on gallbladder cancer cells and identify the mechanisms underlying its potential anticancer effects. We showed that Sch B inhibited the viability and proliferation of human gallbladder cancer cells in a dose-, time -dependent manner through MTT and colony formation assays, and decrease mitochondrial membrane potential (Delta Psi m) at a dose-dependent manner through flow cytometry. Flow cytometry assays also revealed G0/G1 phase arrest and apoptosis in GBC-SD and NOZ cells. Western blot analysis of Sch B-treated cells revealed the upregulation of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP and downregulation of Bcl-2, NF-kappa B, cyclin D1 and CDK-4. Moreover, this drug also inhibited the tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data demonstrated that Sch B induced apoptosis in gallbladder cancer cells by regulating apoptosis-related protein expression, and suggests that Sch B may be a promising drug for the treatment of gallbladder cancer.
引用
收藏
页码:13235 / 13250
页数:16
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